Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Salviae Miltiorrhzae Radix on Cultured Spinal Dorsal Root Ganglion Neurons Damaged by Reactive Oxygen Species

활성산소로 손상된 척수후근신경절세포에 대한 난참의 효과

  • Seo Eun A (College of Life Science, Wonkwang University) ;
  • Choi Yu Sun (School of Medicine, Wonkwang Medical Science, Wonkwang University) ;
  • Yang Hyun Woong (School of Medicine, Wonkwang) ;
  • Lee Kang Chang (Professional Graduate School of Oriental Medicine)
  • 서은아 (원광대학교 생활과학대학) ;
  • 최유선 (원광대학교 의과대학, 원광의과학연구소) ;
  • 양현웅 (원광대학교 의과대학) ;
  • 이강창 (원광대학교 한의학 전문대학원)
  • Published : 2003.10.01
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Abstract

To evaluate the neurotoxicity of reactive oxygen species (ROS) in cultured cultured spinal dorsal root(DRG) neurons derived from neonatal mouse, Cytotoxicity was measured by MTS assay after cultured cells were grown for 3 hours in the media containing 1～60 μM hydrogen peroxide (H₂O₂). In addition the neuroprotective effect of Salviae Miltiorrhzae Radix (SMR) was measured in these cultrures. Cell viability was positively decreased in a dose- and time-dependent manner after exposure of cultured mouse DRG neurons to 30 tt M H202 for 3 hours. In the neuroprotective effect of SMR on H₂O₂-mediated toxicity, SMR prevented the H₂O₂-induced neurotoxicity in these cultures. From these results. it suggests that H₂0₂ is toxic in cultured mouse spinal motor neurons and selective herb extract such as Uncariae Ramulus Cum Uncis is effective in prevetion of the neurotoxicity induced by H₂O₂.

Keywords

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