Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Squalene on the Mice Hepatotoxicity Induced by Lead Acetate

납으로 유발된 생쥐 간장 독성에 대한 스쿠알렌의 효과

  • Kim, Jong-Se (Dept. of Biology, College of Natural Science, Chosun University)
  • 김종세 (조선대학교 자연과학대학 생물학과)
  • Published : 2003.09.01
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Abstract

To investigate the effect of the squalene against the lead toxicity. A healthy male of ICR mice were used for experiment. The SOD was observed after the intraperitoneal injection in mice. The ultrastructural changes of the liver were observed after the intraperitoneal injection in mice. The experimental groups were divided into two groups. Group A was control group that squalene was not treated after intraperitoneal injection of lead acetate. Group B was squalene treatment group that squalene solution was injected after intraperitoneal injection of lead acetate. All groups were used to 10 mice. The results were as follow: SOD activity in the liver, Group A was lower than in normal. But, Group B was higher than in Group A (P<0.05). In the histological observation, Group A were showed that the inner cavity of mitochondria swellen and development of cristae weakened. Swelling of lamellae of rough endoplasmic reticulum (rER) was showed. It was concluded that the SQ will be effective for the recovery of hepatic cell at lead intoxication.

본 연구의 목적은 스쿠알렌이 납 중독에 대해 효과가 있는 지를 관찰하고자 하였다. 건강한 ICR계 생쥐를 사용하였다. 납을 복강 투여한 후 SOD와 간 조직의 미세구조를 관찰하였다. 실험군은 다음과 같다. Group 1은 납을 복강 투여한 후 스쿠알렌을 처치하지 않은 대조군, Group 2는 납을 복강 투여한 후 스쿠알렌을 처치한 군으로 각 실험군당 생쥐 10마리를 사용하였다. 실험 결과는 다음과 같다. SOD 측정 결과 대조군은 정상군에 비해 수치가 훨씬 낮게 나타났지만, 스쿠알렌을 처치한 군에서는 정상군에 비해 낮지만, 대조군 보다 수치가 높게 나타남을 관찰할 수 있었다(P<0.05). 간 조직의 미세구조에의 경우 납 단독 처치군의 경우사립체의 팽대와 rER은 팽대 및 절단된 상태가 관찰되었으나, 스쿠알렌을 처치한 군에서는 납 단독 처치군에 비해 손상 정도가 덜하였다. 본 연구를 통해 스쿠알렌이 납 중독에 의한 간장 조직의 손상 회복에 대해 효과가 있는 것으로 생각되어진다.

Keywords

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