The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Anti-Apoptotic Effect of Rheum undulatum Water Extract in Pancreatic ${\beta}-cell$ Line, HIT-T15

  • Yoon, Seo-Hyun (Laboratory of Pharmacology, School of Pharmacy, Kyung Hee University) ;
  • Hong, Mee-Sook (Department of Pharmacology, Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
  • Chung, Joo-Ho (Department of Pharmacology, Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
  • Chung, Sung-Hyun (Laboratory of Pharmacology, School of Pharmacy, Kyung Hee University)
  • Published : 2004.02.21
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Abstract

Sopungsungi-won has been used as a traditional medicine for diabetes and it has been proved to be a potential remedy for type 2 diabetes mellitus. We previously reported that water extract of Sopungsungi-won exhibits anti-diabetic effects both in vivo and in vitro experiments. In the present study, we have chosen to examined anti-apoptotic effect of Rheum undulatum, which is the main component of Sopungsungi-won, on pancreatic ${\beta}-cells$, HIT-T15, against hydrogen peroxide $(H_2O_2)$. oxidative stress. To investigate the anti-apoptotic effect of Rheum undulatum water extract (RUWE) against $H_2O_2-induced$ apoptosis in pancreatic ${\beta}-cell$ line of hamster, HIT-T15, MTT assay, DAPI staining, TUNEL assay, RT-PCR and caspase-3 enzyme assay were performed. The morphological analysis demonstrated that cells treated with $H_2O_2$ exhibited classical apoptotic features, while such changes was reduced in cells pre-treated with RUWE. In addition, RUWE pre-treated cells prior to $H_2O_2$ treatment induced increase of levels of bcl-2 expression and decrease of caspase-3 enzyme activity compared to cells treated with $H_2O_2$ only. These results provide the possibility of usage of RU in patients with progressively deteriorated diabetes.

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References

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