Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Ramulus et Uncus Uncariae DM fraction on CT105-injuried Neuronal Cells

조구슬 디클로로메탄분획이 CT105에 의한 신경세포 상해에 미치는 영향

  • Jang Hyun Ho (Department of Neuropsychology, College of Oriental Medicine, Wonkwang University) ;
  • Choi Hyuk (Department of Neuropsychology, College of Oriental Medicine, Wonkwang University) ;
  • Yang Hyun Duk (Department of Neurological Medicine, Wonkwang University SanBon Medical Center) ;
  • Kim Sang Tae (JuAm of Research Institute Bioscience & Biotechnology) ;
  • Kim Tae Heon (Department of Neuropsychology, College of Oriental Medicine, Wonkwang University) ;
  • Kang Hyung Won (Department of Neuropsychology, College of Oriental Medicine, Wonkwang University) ;
  • Lyu Young Su (Department of Neuropsychology, College of Oriental Medicine, Wonkwang University)
  • 장현호 (원광대학교 한의과대학 신경정신과학교실) ;
  • 최혁 (원광대학교 한의과대학 신경정신과학교실) ;
  • 양현덕 (원광대학교 산본병원 신경과) ;
  • 김상태 (주암생명공학연구소) ;
  • 김태현 (원광대학교 한의과대학 신경정신과학교실) ;
  • 강형원 (원광대학교 한의과대학 신경정신과학교실) ;
  • 유영수 (원광대학교 한의과대학 신경정신과학교실)
  • Published : 2004.12.01
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Abstract

The purpose of this study was to estimate the effects of Ramulus et Uncus Uncariae DM fraction on CT105-injuried neuronal cells. We were examined by ROS formation, neurite outgrowth assay and DPPH scravage assay. Additionally, we investigated the association between the CT105 and neurite degeneration caused by CT105-induced apoptotic response in neurone cells. We studied on the regeneratory and inhibitory effects of anti-Alzheimer disease in pCT105-induced neuroblastoma cell lines by REUD. Findings from our experiments have shown that REUD inhibits the synthesis or activities of CT105, which has neurotoxityies and apoptotic activities in cell line. In addition, treatment of REUD(>50㎍/㎖ for 12 hours) partially prevented CT105-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by the treatment with its. REUD(>50㎍/㎖ for 12 hours) repaired CT105-induced neurite outgrowth when SK-N-SH cell lines was transfected with CT105. As the result of this study, In REUD group, the apoptosis in the nervous system was inhibited, the repai: against the degeneration of Neuroblastoma cells by CT105 expression was promoted. Base on these findings, REUD may be beneficial for the treatment of AD.

Keywords

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