Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cytotoxicity Effects of Fraction and Chloroform Extracts from Corn is fructus on Cancer Cell Lines

산수유 클로로포름 추출물과 분획물의 암세포주에 대한 세포독성

  • Yang Hyun Ok (Department of Cosmetics, Wonkwang Hearth Science College) ;
  • Choi Won Hyung (Department of Herbal Rresources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Young Hyun (Division of Natural Science of Natural Sciences College, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baek Seung Hwa (Department of Herbal Rresources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chun Hyun Ja (Division of Natural Science of Natural Sciences College, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 양현옥 (원광보건대학교 미용피부관리과) ;
  • 최원형 (한약자원 개발학과) ;
  • 김용현 (원광대학교 자연과학기술학부ㆍ원광대학교 한의학전문대학원) ;
  • 백승화 (한약자원 개발학과) ;
  • 천현자 (원광대학교 자연과학기술학부ㆍ원광대학교 한의학전문대학원)
  • Published : 2004.10.01
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Abstract

Cornis fructus were extracted by successive extractions and then fractionated with chloroform extract to get active fractions. This study was performed to determine the cytotoxic effect of chloroform extract from Corn is fructus on NIH 3T3 fibroblasts and cancer cell lines using MTT assay. All extracts did not exhibit cytotoxicity in NIH 3T3 fibroblasts. Chloroform extract exhibited antitumor activity in A549, MDA-MB-123, B16 melanoma and SNU-C4 cells. Futher fractionation with chloroform extract was performed to obtain effective fractions. 3 fraction showed the strongest cytotoxic effect against A549, MDA-MB-123, B16 melanoma and SNU-C4 cells. These results suggest that 3 fraction of the chloroform extract from Cornis fructus possessed bioactive material of antitumorous agents.

Keywords

References

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