Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Modulation of Bee Venom on Th1/Th2 Cell Lineage Development

봉독 추출액이 helper T cell 분화에 미치는 영향

  • Ko Eun Jung (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Nam Sang Soo (Department of Acupuncture and Moxibustion, College of Oriental Medicine, KyungHee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, KyungHee University, Purimed R&D Institute)
  • 고은정 (경희대학교 한의과대학 생리학교실) ;
  • 남상수 (경희대학교 한의과대학 침구학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드㈜ 기업부설연구소)
  • Published : 2004.10.01
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Abstract

In this study, the immunological effect of a traditional Korea herbal acupuncture, that has been widely used for the treatment of various immunological disorders including inflammation in Korea, was examined in vitro and in vivo. In our previous study demonstrated that BV increased the expression of IFN-γ mRNA, that plays pivotal role in T cell response. This study was designated to evaluate the effect of BV on helper T cell development by monitoring Th1/Th2 specific cytokine secretion patterns in artificially induced Th1/Th2 polarized condition and in vivo. The results demonstrated that BV didn't have mitogenic effects on the unstimulated CD4+ T cell, but increased the CD4+ T cell proliferation upon activation with anti-CD3/CD28 antibody. The Th1 cells were over-populated dramatically in Th1 driven condition with BV treatment, while the Th2 cells were increased slightly in Th2 skewed condition. Furthermore, under Th1-skewed conditions, the level of IFN-γ was considerably increased with BV treatment. Besides, the expression of T-bet, a transcription factor that plays pivotal role in Th1 lineage programming, was increased with BV treatment. The expressions of IFN-γ and T-bet were also significantly increased in vivo. The results that Th1 specific cytokine secretion were considerably increased and Th2 specific cytokine secretion were not significantly changed in vitro and in vivo indicated that BV enhances Th1 lineage development, Therefore, these results suggest that BV might be desirable agent for correction of Th1 dominant pathological disorders.

Keywords

References

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