Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Induction of Apoptotic Cell Death by Healthful Decoction Utilizing Phellinus Linteus in Human Lung Carcinoma Cells

상황을 이용한 보건기능 개선제의 인체폐암세포 apoptosis 유발에 관한 연구

  • Park Cheol (Department of Biochemistry, Dongeui University) ;
  • Lee Yong Tae (Department of Physiology, Dongeui University) ;
  • Kang Kyung Hwa (Department of Physiology, Dongeui University) ;
  • choi Byung Tae (Department of Anatomy, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine) ;
  • Jeong Young Kee (Department of Microbiology. College of Natural Sciences, Dongeui University) ;
  • Choi Yung Hyun (Department of Biochemistry, Dongeui University)
  • 박철 (동의대학교 한의과대학 생화학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 최병태 (동의대학교 해부학교실 및 한의학연구소) ;
  • 정영기 (동의대학교 자연과학대학 미생물학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실)
  • Published : 2004.06.01
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Abstract

In the present study, we investigated the effects of aqueous extract of the healthful decoction utilizing Phellinus linteus (HDPL) on the cell growth of human lung carcinoma tumor cell line A549. Exposure of A549 cells to HDPL resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometric analysis. This increase in apoptosis was associated with inhibition and/or degradation of apoptotic target proteins such as poly(ADP-ribose) polymerase (PARP), b-catenin and phospholipase C- 1 (PLC- 1) protein. HDPL treatment induced the down-regulation of anti-apoptotic Bcl-2 expression, an anti-apoptotic gene, however, the level of Bax. a pro-apoptotic gene, was increased by HDPL treatment. In addition, HDPL-induced apoptotis of A549 cells was connected with activation of caspase-3 and caspase-9 protease in a dose-dependent manner, however, the levels of inhibitor of apoptosis proteins family were remained unchanged. Taken together, these results indicated that the anti-proliferative effects of HDPL were associated with the induction of apoptotic cell death through regulation of several major growth regulatory gene products such as Bcl-2 family expression and caspase protease activity, and HDPL may have therapeutic potential in human lung cancer.

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References

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