Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Contents of Homogentisic acid and 3,4-Dihydroxybenzaldehyde in the Pinellia ternata by Various Processing Method and Its Safety Estimate

포제방법에 따른 반하의 Homogentisic acid와 3,4-dihydroxybenzaldehyde 함량 및 안정성 평가

  • Han Jong Hyun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Jo Sung Gyun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Lee Mi Jeong (Department of Herbal Resource,. Professional Graduate School of Oriental Medicine) ;
  • Baek Seung Hwa (Department of Food Science & Biotechnology, Chungbuk Provincial University of Science & Technology) ;
  • Park Sung Hye (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
  • 한종현 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 조성균 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이미정 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백승화 (충북도립대학 식품생명과학과) ;
  • 박성혜 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2004.06.01
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Abstract

This study was carried out for establishment of toxicological monitoring system in oriental medicinal plants. Hence on our research, we used Banha(Pinellia ternata) and Kangbanha, Bubbanha, Jaebanha, Geokbanha by distinguished processing methods. These are frequently used in Bangyakhabpeon, which is one of the most well-known clinical book in oriental medicine. As we reviewed the reported documents, we judge homogentisic acid(HA) and 3,4-dihydroxybenzaldehyde(3,4-DBA) as poisonous substance and to verify its existence, we established analysis condition of HPLC by gaining sequential fraction extracts and studied the degree of its virulence to provide basic information on the guidelines of using this medicine. Optimum condition of HPLC was H₂O : MeOH : CH₃COOH (57:35:8) in HA and 3,4-DBA analysis. HA content of raw Banha was 11.03mg/100g and HA contents of its processed product were decreased. Exceptionally, Jaebanha was increased in 175.97% than raw Banha. 3,4-DBA content of raw Banha was 2.93mg/100g and 3,4-DBA contents of its processed product were decreased. These results will be applies in intake guideline establishment, quality control and stability evaluation of oriental medicinal plants.

Keywords

References

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