Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Ultrastructure of the Gill of the Parrot fish, Oplegnathus fasciatus

돌돔 (Oplegnathus fasciatus) 아가미의 미세구조

  • Kim, Jae-Won (Department of Marine Biology, Pukyong National University) ;
  • Baeck, Gun-Wook (Korea Inter-university Institutes of Ocean Science, Pukyong National University)
  • 김재원 (부경대학교 해양생물학과) ;
  • 백근욱 (부경대학교 해양과학공동연구소)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The ultrastructure of the gills of Oplegnathus fasciatus was examined by means of light and transmission electron microscopes. The gills have primary and secondary filaments (lamellae). The following cells are identified and described : epithelial cell, pillar cell, chloride cell and mucose cell. The simple epithelial layer consists of squamous epithelium containing a large nucleus and the surface is covered with some of microridges. The lamella pillar structures are characterized by axial microtubules and lateral membrane interdigitations. Chloride cells contain a lot of mitochondria and specifically developed tubular systems. The rough endoplasimic reticulum and golgi complex, and some of mucous granules were observed in immature mucous cells. The mature mucous cells were AB-PAS positive, globular in shape, and had mucous granules of similar size with various electron densities.

돌돔 아가미의 미세구조를 광학현미경과 투과전자현미경을 이용하여 관찰하였다. 아가미는 새궁 앞, 뒤 2열로 된 빗모양의 많은수의 새엽과 그리고 각 새엽은 전후 두 개의 새판(gill lamellae)이 근접하여 2열로 배열되어 있다. 상피세포층은 단층으로 큰 핵을 가진 편평상피로 구성되고 표면에는 미세융기가 잘 발달되어 있다. 새판의 기둥 구조는 세로 방향의 미세소관들과 측면 membrane interdigitation을 가지며, 염세포는 수많은 미토콘드리아와 tubular system이 잘 발달되어 있다. 미성숙된 점액세포는 조면세포체와 골지체가 잘 발달되었으며 일부 발달된 점액 과립을 가지고 있다. 성숙한 점액세포는 AB-PAS에 푸른 색을 띄었으며 구형이고 다양한 전자밀도를 가진 거의 같은 크기의 점액과립을 가지고 있다.

Keywords

References

  1. Andrew W, Hickman CP: Histology of the vertebrates. Mosby Co., Saint Louis, pp. 112 114, 1974
  2. Chyung MK: The fishes of Korea. IIji sa, Seoul, pp. 727, 1977
  3. Fletcher TC, Jones R, Reid L: Identification of glycoproteins in goblet cells of epidermis and gill of plaice (Pleuronectes platessa L.), flounder (Platichthys flesus L.) and rainbow trout (Salmo gairdneri Richardson). Histochem J 8 : 597 608, 1976 https://doi.org/10.1007/BF01003961
  4. Handy RD, Eddy FB: The absence of mucus on the secondary lamellae of unstressed rainbow trout, Oncorhynchus mukiss (Walbaum). J Fish BioI 38 : 153 155, 1991 https://doi.org/10.1111/j.1095-8649.1991.tb03100.x
  5. Huh MD: The histological structure and the pathological lesions of gill in teleosts. J Fish PathoI 6(1) : 65 70, 1993
  6. Jakowska S: Mucus secretion in fish a note. Ann NY Acad Sci 106 : 458 462, 1963 https://doi.org/10.1111/j.1749-6632.1963.tb16658.x
  7. Kang WS, Moon YH, Han JW, Kim HH: Apoptosis in chloride cells of killifish(Orizias latipes) gills adapted to the seawater. Korean J Electron Microscopy 26(3) : 369 377, 1996
  8. Karlsson L: Gill morphology in the zebrafish, Brachydanio rerio (Hamilton Buchanan). J Fish BioI 23 : 511 524, 1983 https://doi.org/10.1111/j.1095-8649.1983.tb02931.x
  9. Kendall MW, Dale JW: Scanning and transmission electron microscopic observations of rainbow trout (Salmo gairdneri) gill. J Fish Res Board Can 36 : 1072 1079, 1979 https://doi.org/10.1139/f79-150
  10. Keys A, Willmer EN: Chloride secreting cells in the gill of fishes with special refernce to the common eel. J Physiol Lond 76 : 368 377, 1932 https://doi.org/10.1113/jphysiol.1932.sp002932
  11. Kim BS: Histological comparison of the organs of some fishes inhabiting in the different water area. Master thesis of Nat Fish Uni Busan, pp. 35, 1992
  12. Kim HH, Chi YD, Moon YW, Kang WS: Ultrastructural changes of chloride cells of the guppy (Poecilia reticulatus) gill according to the environmental salinity. Korean J Zool 36 : 264 275, 1993
  13. Laurent P, Dunel S: Morphology of gill epithelia in fish. Am J Physiol 238 : 147 159, 1980
  14. Lee YC, Chang YJ, Lee BK: Osmoregulation capability of juvenile grey mullets (Mugil cephalus) with the different salinities. J Korean Fish Soc 30 : 216 224, 1997
  15. Lubin RT, Rourke AW, Bradley TM: Ultrastructural alterations in branchial chloride cells of Atlantic salmon, Salmo salar, during parr smolt transformation and early development in sea water. J Fish BioI 34 : 259 272, 1989 https://doi.org/10.1111/j.1095-8649.1989.tb03307.x
  16. Mallat J: Fish gill structural changes induced by toxicants and other irritants: a statistical review. Can J Fish Aquat. Sci 42 : 630 648, 1985 https://doi.org/10.1139/f85-083
  17. Moon YW: Ultrastructural and histochemical changes of mucous cells in the gill epithelium of the seawater adapted guppy (Poecilia reticulatus). Korean J. Zool 38 : 570 580, 1995
  18. Payan P, Girard JP, Mayer Gostan N: Branchial ion movements in teleosts: the roles of respiratory and chloride cells. In: Hoar WS, Randall DJ, ed, Fish Physiology, XB, pp. 36 63, Academic Press, New York, 1984
  19. Perera KML: Ultrastructure of the primary gill lamellae of Scomber australasicus. J Fish BioI 43 : 45 59, 1993
  20. Temmink JHM, Bouwmeister PJ, De Jong P, Van Den Berg JHJ: An ultrastructural study of chromate induced hyperplasia in the gill of rainbow trout (Salmo gairdneri). Aquat. Toxicol 4 : 165 179, 1983 https://doi.org/10.1016/0166-445X(83)90053-X
  21. Yoon JM: Ultrastructural change of osmoregulatory cells during seawater adaptation in rainbow trout (Oncorhynchus mykiss). Korean J IchthyoI 12(2) : 111 117, 2000
  22. Zenker WGE, Ferguson HWI, Baeker K, Woodward B: Epithelial and pillar cell replacement in gills of juvenile trout, Salmo gairdneri Richardsin. Comp Biochem Physiol 86A : 423 428, 1987 https://doi.org/10.1016/0300-9629(87)90518-4