동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제21권3호
  • /
  • Pages.658-665
  • /
  • 2007
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

맹종죽의 항산화활성을 통한 항고혈압 효능

Anti-hypertensive Effects of ethanol extract of Phyllostachys Pubescens via Antioxidant Activity

  • 이혜숙 (동국대학교 한의과대학 생리학교실) ;
  • 박민희 (인제대학교 의생명공학대학 식품생명과학부 바이오헬스소재연구센터 식품과학연구소) ;
  • 김정숙 (인제대학교 의생명공학대학 식품생명과학부 바이오헬스소재연구센터 식품과학연구소) ;
  • 임병우 (건국대학교 의료생명대학 생명과학부) ;
  • 문갑순 (건국대학교 의료생명대학 생명과학부) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Lee, Hye-Suk (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Park, Min-Hee (School of Food and Life Science, Biohealth Products Research Center and Food Science Institute, Inje University) ;
  • Kim, Jung-Suk (School of Food and Life Science, Biohealth Products Research Center and Food Science Institute, Inje University) ;
  • Lim, Beong-Ou (Department of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Moon, Gap-Soon (Department of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 발행 : 2007.06.25
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초록

Phyllostachys pubescens (Maengiong-Juk), a kind of the bamboo, was reported to have many beneficial pharmacological actions. in this study, of using 70% ethanol extract of Phyllostachys pubescens we investigated its efficacy on angiotensin converting enzyme (ACE) and antioxidant enzyme activities. In addition, vasorelaxant effect was examined in rat aortic rings. The inhibitory effect of ACE activity by Phyllostachys pubescens extract (PPE) was dose-dependently increased by 61.42% at 10mg/ml. PPE relaxed the pre-contracted rat aortic rings with 10$^{-6}$M phenylephrine, showing about 88% at 4.0mg/ml. Sprague Dawley (SD) rats were given different concentrations of PPE mixed in the drinking water for 10 weeks. PPE did not show any difference with control group in blood pressure, body weight (BW) and food intake. However, it revealed the highest total antioxidative effect at dose of 1.0 g/100 g BW in plasma by TEAC assay. Thiobarbituric acid reactive substance (TBARS) and protein carbonyl levels which are markers of tissue peroxidation, were significantly lowed at the same dosage. Furthermore, hepatic antioxidant enzymes such as total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and catalase activities were also significantly increased by PPE (1.0 g/100 g BW). In conclusion, we suggest that PPE might have antihypertensive effect through increasing antioxidant activities.

키워드

참고문헌

  1. Food Nutrients Dictionary. J. Korean Soc. Food Sci. Nutr., p 821, 1998
  2. Samy, I.M., Ashok, K., James, R.S. Mechanisms by Which Angiotensin-Converting Enzyme Inhibitors Prevent Diabetes and Cardiovascular Disease, Am. J. Cardiol., 91: 30H-37H, 2003
  3. Brewster, U.C., Setaro, J.F., Perazella, M.A. The renin- angiotensin-aldosterone system; cardiorenal effects and implications for renal and cardiovascular disease states: Am. J. Med. Sci., 326: 15-24, 2003 https://doi.org/10.1097/00000441-200307000-00003
  4. Tummala, P.E., Chen, X.L., Sundell, C.L., Laursen, J.B., Hammes, C.P., Alexander, R.W., Harrison, D.G., Medford, R.M. Angiotensin II induces vascular cell adhesion molecule-1- expression in rat vasculature; a potential link between the renin-angiotensin system and atherosclerosis. Circulation, 100: 1223-1229, 1999 https://doi.org/10.1161/01.CIR.100.11.1223
  5. Hara, Y., Matsuzaki, T., Suzuki, T. Angiotensin I converting enzyme inhibiting activity of tea components. Nippon Shokuhin Kogyo Gakkashi, 61: 803-808, 1987
  6. 홍상필, 김명희, 오세욱, 한찬규, 김용현. Chitosan 올리고당의 안지오텐신 전환효소 활성 억제 및 SHR에서의 고혈압 억제 특성. 한국식품과학회지 30: 1476-1479, 1998
  7. 박건구, 류재원, 최은경, 노환성. SHR(Spontaneously Hypertensive Rat)를 이용한 송엽, 익모초 추출물의 항고혈압 작용. 응용약물학회지 8: 27-31, 2000
  8. Zalba, G., San, J.G., Moreno, M.U., Fortuno, M.A., Fortuno, A., Beaumont, F.J., Diez, J. Oxidative stress in arterial hypertension. Role of NAD(P)H oxidase. Hypertension 38: 1395-1399, 2001 https://doi.org/10.1161/hy1201.099611
  9. Elena, M.V., Barbara, P., Cesar, G.F. Concerted action of the renin-angiotensin system, mitochondria, and antioxidant defenses in aging. Molecular Aspects of Medicine 25: 27-36, 2004 https://doi.org/10.1016/j.mam.2004.02.006
  10. 백종원. 국내산 대나무의 항균활성과 식품보존효과. 이학석사학위논문, 2003
  11. 홍남두, 김종우, 최승기, 김남재, 손정곤. 죽력의 약리작용에 관한 연구 (제1보). 경희약대논문집, 10: 69-75, 1982
  12. 김낙구, 조숙현, 이상대, 류재산, 심기환. 대나무 열수추출물의 화학적 특성. 한국식품저장유통학회 8(4):469-474, 2001
  13. 이민자, 문갑순. 한국산 왕대, 솜대, 맹종죽, 조릿대 및 오죽의 항산화 효과. 한국식품과학회지 35: 1226-1232, 2003
  14. 이영노. 한국식물도감(FLORA of Korea). 교학사, pp 970-971, 2002
  15. 이민자, 김은영, 문갑순. 맹종죽 추출물 코팅쌀 식이가 고콜레스테롤 섭취 토끼의 항산화 시스템에 미치는 영향. 한국식품영양과학회지 33: 973-980, 2004
  16. 김은영, 이민자, 송영옥, 문갑순. 맹종죽(Phyllostachys Pubescens) 추출물 코팅쌀이 Atherogenic 식이를 섭취한 C57BL/6 마우스의 항산화 시스템에 미치는 영향. 대한지역사회영양학회지, 9:536-544, 2004
  17. Cushman, D.W., Cheung, H.S. Spectrophotometric assay and properties of angiotensin converting enzyme of rabbit lung. Biochem. Pharm. 20: 1637-1647, 1971 https://doi.org/10.1016/0006-2952(71)90292-9
  18. Marklund, S., Marklund, G. Involvement of the superoxide anion radical in the autpxodatopm of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 47: 469-474, 1974 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  19. Lawrence, R.A., Burk, F. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71: 952-958, 1976 https://doi.org/10.1016/0006-291X(76)90747-6
  20. Inger, C., Bengt, M. Methods in enzymology. Academic press. Inc. 113: 484-490, 1985
  21. Aebi, H. Catalase in vitro. Methods in Enzymology, 105:121-126, 1984 https://doi.org/10.1016/S0076-6879(84)05016-3
  22. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology & Medicine 26: 1231-1237, 1999 https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Ohkawa, H., Ohishi, N., Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351, 1979
  24. Oliver, C.N., Ahn, B., Moerman, E.J., Goldstein, S., Stadtman, E.R.. Age-related changes in oxidized proteins. J. Biol. Chem. 262: 5483-5492, 1987
  25. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  26. Stewart, J.M., Ferreira, S.H., Greene, L.J. Bradykinin potentiating peptide PCA-Lys-Trp-Ala-Pro. An inhibitor of the pulmonary inactivation of bradykinin and conversion of angiotensin I to II. Biochem. Pharmacol. 20(7):15557-15567, 1971
  27. Soffer, R.L. Angiotensin-converting enzyme and the regulation of vasoactive peptides. Annu. Rev. Biochem. 45: 73-94, 1976 https://doi.org/10.1146/annurev.bi.45.070176.000445
  28. Ohara, T., Ohinata, H., Muramatsu, N. Enzymatic degradation of rutin in processing of buckwheat noodles. Nippon Shokuhin Kogyo Gakkashi 36: 121, 1989
  29. 나창수, 윤대환, 최동희, 김정상, 조춘화, 은종방. 배 추출 펙틴이 2K1C 고혈압 흰쥐의 혈압, 혈장 Renin, ANP 및 Cardiac Hypertrophy에 미치는 영향. 한국식품영양과학회지 32: 700-705, 2003
  30. 김현수, 인영민, 정석근, 함준상. 자연발증고혈압쥐에서 카제인 가수분해물의 혈압강하효과. 동물자원지 44: 483-490, 2002
  31. 김미정, 변명우, 장명숙. 대나무(신의대)잎의 생리활성 및 항균성 효과. 한국영양식량학회지 25: 135-142, 1996
  32. Lee, S.K. Antimicrobial Activity of Bamboo (Phyllostachys bambusoides) Essential Oil. J. Fd Hyg. Safety.15(1):55-59, 2000
  33. 이민자, 김은영 정근옥, 박건영, 문갑순. 한국산 대나무의 항돌연변이 효과 및 대나무 코팅쌀의 간 독성 억제효과. 한국식품영양과학회지 33(8):1279-1285, 2004
  34. Floyd, R.A. Neuroinflammatory processes are important in neurodegenerative diseases: an hypothesis to explain the increased formation of reactive oxygen and nitrogen species as major factors involved in neurodegenerative disease development. Free Radic Biol Med. 26(9-10):1346-1355, 1999
  35. Kojda, G., Harrison, D. Interactions between NO and reactive oxygen species: pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc Res. 43(3):562-571, 1999 https://doi.org/10.1016/S0008-6363(99)00169-8
  36. Guzik, T.J., West, N.E., Black E, McDonald D, Ratnatunga C, Pillai R, Channon KM. Vascular superoxide production by NAD(P)H oxidase: association with endothelial dysfunction and clinical risk factors. Circ Res. 86(9):E85-90, 2000 https://doi.org/10.1161/01.RES.86.9.e85
  37. Panza, J.A., Quyyumi, A.A., Brush, J.E. Jr, Epstein, S.E. Abnormal endothelium-dependent vascular relaxation in patients with essential hypertension. N Engl J Med. 323(1): 22-27, 1990 https://doi.org/10.1056/NEJM199007053230105
  38. Yannik, M., Robert, B. Repression of gene expession by oxidative stress. Biochem. J. 342: 481-496, 1999 https://doi.org/10.1042/0264-6021:3420481
  39. Wink, D.A., Michell, J.B. Chemical biology of nitric oxide; Insight into regulatory, cytotoxic, and cytoprotective mechanism of nitric oxide. Free Rad Biol Med. 25: 434-456, 1998 https://doi.org/10.1016/S0891-5849(98)00092-6
  40. Kehrer, J.P. Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol. 23(1):21-48, 1993 https://doi.org/10.3109/10408449309104073
  41. 김영국. 식이에 의한 간지질 및 효소 활성 변화에 관한 연구. 한국영양학회지 6(1):15-29, 1973