허혈 상태의 PC12 세포에 대한 대보원전(大補元煎)의 신경보호효과

Neuroprotective Effects of Daebowonjeon on PC12 Cells Exposed to Ischemia

  • 김봉상 (원광대학교 한의과대학 내과학교실) ;
  • 이선우 (원광대학교 한의과대학 내과학교실, 원광대학교 한의학전문대학원) ;
  • 문병순 (원광대학교 한의과대학 내과학교실, 원광대학교 한의학전문대학원)
  • Kim, Bong-Sang (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Sun-Woo (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2007.02.25

초록

Neuronal ischemia is a pathological process caused by a lack of oxygen (anoxia) and glucose (hypoglycemia), resulting in neuronal death. It is believed that apoptosis is one of the mechanisms involved in ischemic cell death. Neuronal apoptosis is a process characterized by nuclear DNA fragmentation, changes of plasma membrane organization. To elucidate the mechanism of neuronal death following ischemic insult and to develop neuroprotective effects of Daebowonjeon(DBWJ) against ischemic damage, in vitro models are used. In vitro models of cell death have been devloped with pheochromocytoma (PC12) cell, which have become widely used as neuronal models of oxidative stress, trophic factor, serum deprivation and chemical hypoxia. Using a special ischemic device and PC12 cultures, we investigated an in vitro model of ischemia based on combined Oxygen and Glucose Deprivation (OGD) insult, followed by reoxygenation, mimicking the pathological conditions of ischemia. In this study, Daebowonjeon rescued PC12 cells from Oxygen-Glucose Deprivation (OGD)-induced cell death in a dose-dependent manner The nuclear staining of PC12 cells clearly showed that DBWJ attenuated nuclear condensation and fragmentation which represent typical neuronal apoptotic characteristics. DBWJ also prevents the LDH release and induction of Hypoxia Inducing Factor (HIF)-1 by OGD-exposed PC12 cells. Furthermore, DBWJ reduced the activation of polyADP-ribose polymerase (PARP) by OGO-exposed PC12 cells. These results suggest that apoptosis is an important characteristic of OGD-induced neuronal death and that oriental medicine, such as DBWJ, may prevent PC12 cell from OG D-induced neuronal death by inhibiting the apoptotic process.

키워드

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