Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Neuroprotective Effect of Hwangryunhaedok-tang Against Glucose-deprivation Induced Cytotoxicity in PC-12 Cells

Glucose-deprivation으로 유발된 PC-12 cell의 세포 고사에 대한 황연해독탕(黃連解毒湯)의 보호 효과

  • Kim, Hye-Yoon (Department of Oriental Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun-Ho (Department of Oriental Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, In (Department of Oriental Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 김혜윤 (원광대학교 한의과대학 심계내과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실)
  • Published : 2008.12.25
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Abstract

The purpose of the study was to confirm what effect HRHDT treatment had on cell extinction by damage of endoplasmic reticulum induced to PC-12 cell damage by glucose deprivation. The study confirmed what effect it had on forming the condition of glucose deprivation within a culture fluid of PC-12 cell and on a nerve cell's survival rates and tested whether HRHDT could prevent extinction of PC-12 cell by glucose deprivation. Also, the study confirmed what effect HRHDT treatment had on the emitted quantity of LDH by glucose deprivation. To examine PC-12 cell's behavioral change under the condition of glucose deprivation and a protective effect of HRHDT on the change, the study observed PC-12 cell's behavioral change with a microscope. Also, the study confirmed density of calcium ion within cells followed by a culture time in the condition of glucose deprivation with FACS and confirmed what effect HRHDT treatment had on the above density of calcium ion within cells. Finally, the study carried out the western blot and confirmed what effect HRHDT treatment had on revelation of GRP 78 and CHOP protein and a segmental type of aspase 12. In this study, HRHDT rescued PC-12 cells from glucose deprivation-induced cell death. HRHDT also prevents the LDH release, Ca++ accumulation, and morphological change, which was associated with the ER stress. Furthermore, HRHDT reduced the expression of ER chaperone (Grp78 and CHOP) proteins by glucose deprivation in PC-12 cells. These results suggest that HRHDT might provide a useful therapeutic strategy in treatment of the neurodegenerative diseases caused by glucose deprivation injuries.

Keywords

References

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