The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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R-type Calcium Channel Isoform in Rat Dorsal Root Ganglion Neurons

  • Fang, Zhi (National Research Laboratory for Pain, Dental Research Institute and Department of Physiology School of Dentistry, Seoul National University) ;
  • Hwang, Jae-Hong (National Research Laboratory for Pain, Dental Research Institute and Department of Physiology School of Dentistry, Seoul National University) ;
  • Kim, Joong-Soo (National Research Laboratory for Pain, Dental Research Institute and Department of Physiology School of Dentistry, Seoul National University) ;
  • Jung, Sung-Jun (Department of Physiology, College of Medicine, Kangwon National University) ;
  • Oh, Seog-Bae (National Research Laboratory for Pain, Dental Research Institute and Department of Physiology School of Dentistry, Seoul National University)
  • Published : 2010.02.28
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Abstract

R-type $Ca_v2.3$ high voltage-activated $Ca^{2+}$ channels in peripheral sensory neurons contribute to pain transmission. Recently we have demonstrated that, among the six $Ca_v2.3$ isoforms ($Ca_v2.3a{\sim}Ca_v2.3e$), the $Ca_v2.3e$ isoform is primarily expressed in trigeminal ganglion (TG) nociceptive neurons. In the present study, we further investigated expression patterns of $Ca_v2.3$ isoforms in the dorsal root ganglion (DRG) neurons. As in TG neurons, whole tissue RT-PCR analyses revealed the presence of two isoforms, $Ca_v2.3a$ and $Ca_v2.3e$, in DRG neurons. Single-cell RT-PCR detected the expression of $Ca_v2.3e$ mRNA in 20% (n=14/70) of DRG neurons, relative to $Ca_v2.3a$ expression in 2.8% (n=2/70) of DRG neurons. $Ca_v2.3e$ mRNA was mainly detected in small-sized neurons (n=12/14), but in only a few medium-sized neurons (n=2/14) and not in large-sized neurons, indicating the prominence of $Ca_v2.3e$ in nociceptive DRG neurons. Moreover, $Ca_v2.3e$ was preferentially expressed in tyrosine-kinase A (trkA)-positive, isolectin B4 (IB4)-negative and transient receptor potential vanilloid 1 (TRPV1)-positive neurons. These results suggest that $Ca_v2.3e$ may be the main R-type $Ca^{2+}$ channel isoform in nociceptive DRG neurons and thereby a potential target for pain treatment, not only in the trigeminal system but also in the spinal system.

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References

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