The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Calcium Sensitization Induced by Sodium Fluoride in Permeabilized Rat Mesenteric Arteries

  • Yang, En-Yue (Department of Pharmacology, Kyungpook National University School of Medicine) ;
  • Cho, Joon-Yong (Department of Thoracic and Cardiovascular Surgery, Kyungpook National University School of Medicine) ;
  • Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Kim, In-Kyeom (Department of Pharmacology, Kyungpook National University School of Medicine)
  • Published : 2010.02.28
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Abstract

It was hypothesized that NaF induces calcium sensitization in $Ca^{2+}$-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with $\beta$-escin and subjected to tension measurement. NaF potentiated the concentration-response curves to $Ca^{2+}$ (decreased $EC_{50}$ and increased $E_{max}$). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascular tension in $Ca^{2+}$-controlled solution at pCa 7.0 or pCa 6.5, but not at pCa 8.0. NaF-induced vasocontraction and $GTP{\gamma}S$-induced vasocontraction were not additive. NaF-induced vasocontraction at pCa 7.0 was inhibited by pretreatment with Rho kinase inhibitors H1152 or Y27632 but not with a MLCK inhibitor ML-7 or a PKC inhibitor Ro31-8220. NaF induces calcium sensitization in a $Ca^{2+}$ dependent manner in $\beta$-escin-permeabilized rat mesenteric arteries. These results suggest that NaF is an activator of the Rho kinase signaling pathway during vascular contraction.

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References

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