Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Fructus Ligustri Lucidi on Tyrosinase and MITF Expressions

여정자 추출물의 Tyrosinase 및 MITF 발현 억제 효과

  • Han, Gyu-Su (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Mun, Yeun-Ja (Research Center of Traditional Korean Medicine)
  • 한규수 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (한국전통의학연구소)
  • Received : 2010.03.19
  • Accepted : 2010.04.13
  • Published : 2010.04.25
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Abstract

The purpose of this study was to investigate the mechanism of Hexane extract of Fructus Ligustri Lucidi (HFLL)-induced regulation of melanogenesis. An apparent down-regulatory effect of tyrosinase activity was observed when B16F10 cells were incubated with HFLL. Interestingly, HFLL did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 cells, whereas kojic acid directly inhibited tyrosinase activity. Regarding protein levels of melanogenic enzymes, the amounts of tyrosinase and tyrosinase-related protein 1 (TRP-1) were decreased by HFLL, while the amount of tyrosinase-related protein 2 (TRP-2) slightly was reduced after incubation with HFLL. Treatment with HFLL was found to down-regulate microphthalmia-associated transcription factor (MITF). These results suggest that HFLL is an effective inhibitor of pigmentation caused by down regulation via MITF, tyrosinase, and TRP-1 expressions.

Keywords

References

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