Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Carthami Flos on Interstitial Cells of Cajal in the Gastrointestinal Tract

홍화가 위장관 카할간질세포에 미치는 효과

  • Song, Ho-Joon (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kim, Jung-A (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Han, Song-Ee (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kim, Hyung-Woo (Division of Pharmacology School of Korean Medicine, Pusan National University) ;
  • Chae, Han (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kim, Byung-Joo (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kwon, Young-Kyu (Division of Longevity and Biofunctional Medicine, Pusan National University)
  • 송호준 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김정아 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 한송이 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김형우 (부산대학교 한의학전문대학원 약물의학부) ;
  • 채한 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 권영규 (부산대학교 한의학전문대학원 양생기능의학부)
  • Received : 2011.04.19
  • Accepted : 2011.07.15
  • Published : 2011.08.25
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Abstract

The purpose of this study is to investigate the effects of Carthami Flos on interstitial cells of Cajal in the gastrointestinal tract. Many regions of the tunica muscularis of the gastrointestinal (GI) tract display spontaneous contraction. These spontaneous contractions are mediated by periodic generation of electrical slow waves. Recent studies have shown that the interstitial cells of Cajal (ICCs) act as pacemakers and conductors of electrical slow waves in gastrointestinal smooth muscles. We investigated the cytotoxicity activity, antioxidant activity, and pacemaking activity. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay and DCFH-DA (2,7-dichlorofluorescein diacetate) method. The effects of Carthami Flos on the pacemaker potentials in cultured ICCs from murine small intestine were investigated by using whole-cell patch-clamp techniques at $30^{\circ}C$. The addition of Carthami Flos (5, 10, $30{\mu}g$/ml) depolarized the resting membrane potentials in a concentration dependent manner. These results suggest that the GI tract can be targets for Carthami Flos, and their interaction can affect intestinal motility.

Keywords

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