Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Hoelen in RANKL-induced Osteoclast Differentiation

파골세포 분화에 복령 추출물이 미치는 영향

  • Cheon, Yoon-Hee (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Kwack, Seong-Cheoul (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Oh, Jae-Min (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Choi, Min-Kyu (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Kim, Jeong-Joong (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Kwak, Han-Bok (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang University) ;
  • Lee, Myeung-Su (Department of Rheumatology & Institute for Skeletal Disease, Wonkwang University) ;
  • Jeon, Byung-Hoon (Department of Pathology, College of Oriental Medicine & Research Center of Traditional Korean Medicine, Wonkwang University) ;
  • Moon, Seo-Young (Division of Anesthesiology and Pain Medicine, Sanbon Medical Center)
  • 천윤희 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 곽성철 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 오재민 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 최민규 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 김정중 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 곽한복 (원광대학교 의과대학 해부학교실 & 골격계질환연구소 및 환경과학연구소) ;
  • 이명수 (원광대학교 의과대학 류마티스내과학교실 및 골격계질환연구소) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실 & 한국전통의학연구소) ;
  • 문서영 (산본병원 마취통증의학과)
  • Received : 2012.05.17
  • Accepted : 2012.06.14
  • Published : 2012.06.25
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Abstract

Osteoporosis is an important public health issue in postmenopausal women. It is a major public health concern and is widely believed that osteoporosis results from imbalance between bone resorption and bone formation. Recently natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Hoelen (scientific name, Poria cocos) is a mushroom that is used in traditional Chinese medicine. Hoelen exhibits anti-inflammatory activity and has a protective effect on tumor progression. However, the effect of hoelen in osteoclast differentiation remains unknown. Thus, we examined the effect of hoelen in receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Hoelen significantly inhibited RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in dose dependent manner without toxicity. Also, we showed that hoelen significantly inhibited the mRNA expression of tartrate-resistant acid phophatase (TRAP) and nuclear factor of activated T cells 1 (NFATc1) in BMMs treated with RANKL. In Particular, hoelen greatly inhibited the protein expression of NFATc1. Ectopic expression of NFATc1 partially reverses hoelen-mediated inhibition of osteoclast differentiation. Taken together, our results demonstrated that hoelen may be useful treatment option of bone-related disease such as osteoporosis, reumatoid arthritis, and periodontitis.

Keywords

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