Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Polymorphism of NLRP3 Gene and Association with Susceptibility to Digestive Disorders in Rabbit

  • Yang, Yu (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Zhang, Gong-Wei (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Chen, Shi-Yi (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Peng, Jin (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Lai, Song-Jia (Institute of Animal Genetics and Breeding, Sichuan Agricultural University)
  • Received : 2012.09.19
  • Accepted : 2012.10.25
  • Published : 2013.04.01
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Abstract

NLR family pyrin domain containing 3 (NLRP3) is a key component of the inflammasome, whose assembly is a crucial part of the innate immune response. The aim of the present study was to evaluate the association between exon 3 polymorphisms of NLRP3 and the susceptibility to digestive disorders in rabbits. In total, five coding single-nucleotide polymorphisms (cSNPs) were identified; all of which are synonymous. Among them, c.456 C> and c.594 G> were further genotyped for association analysis based on case-control design (n =162 vs n =102). Meanwhile, growing rabbits were experimentally induced to digestive disorders by feeding a fiber-deficient diet, subsequently they were subjected to mRNA expression analysis. Association analysis revealed that haplotype H1 (the two cSNPs: GT) played a potential protective role against digestive disorders (p<0.001). The expression of NLRP3 in the group $H1HX_1$ ($H1HX_1$ is composed of H1H1, H1H3 and H1H4) was the lowest among four groups which were classified by different types of diplotypes. Those results suggested that the NLRP3 gene was significantly associated with susceptibility to digestive disorders in rabbit.

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References

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