The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Lutein on L-NAME-Induced Hypertensive Rats

  • Received : 2013.05.06
  • Accepted : 2013.07.01
  • Published : 2013.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the antihypertensive effect of lutein on $N^G$-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertensive rats. Daily oral administration of L-NAME (40 mg/kg)-induced a rapid progressive increase in mean arterial pressure (MAP). L-NAME significantly increased MAP from the first week compared to that in the control and reached $193.3{\pm}9.6$ mmHg at the end of treatment. MAP in the lutein groups was dose-dependently lower than that in the L-NAME group. Similar results were observed for systolic and diastolic blood pressure of L-NAME-induced hypertensive rats. The control group showed little change in heart rate for 3 weeks, whereas L-NAME significantly reduced heart rate from $434{\pm}26$ to $376{\pm}33$ beats/min. Lutein (2 mg/kg) significantly prevented the reduced heart rate induced by L-NAME. L-NAME caused hypertrophy of heart and kidney, and increased plasma lipid peroxidation four-fold but significantly reduced plasma nitrite and glutathione concentrations, which were significantly prevented by lutein in a dose-dependent manner. These findings suggest that lutein affords significant antihypertensive and antioxidant effects against L-NAME-induced hypertension in rats.

Keywords

References

  1. Feletou M, Vanhoutte PM. Endothelial dysfunction: a multifaceted disorder (The Wiggers Award Lecture). Am J Physiol Heart Circ Physiol. 2006;291:H985-H1002. https://doi.org/10.1152/ajpheart.00292.2006
  2. Taddei S, Virdis A, Mattei P, Ghiadoni L, Sudano I, Salvetti A. Defective L-arginine-nitric oxide pathway in offspring of essential hypertensive patients. Circulation. 1996;94:1298-1303. https://doi.org/10.1161/01.CIR.94.6.1298
  3. Shin W, Cuong TD, Lee JH, Min B, Jeon BH, Lim HK, Ryoo S. Arginase inhibition by ethylacetate extract of Caesalpinia sappan lignum contributes to activation of endothelial nitric oxide synthase. Korean J Physiol Pharmacol. 2011;15:123-128. https://doi.org/10.4196/kjpp.2011.15.3.123
  4. Jacques-Silva MC, Nogueira CW, Broch LC, Flores EM, Rocha JB. Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacol Toxicol. 2001;88:119-125. https://doi.org/10.1034/j.1600-0773.2001.d01-92.x
  5. Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991;43: 109-142.
  6. Radomski MW, Palmer RM, Moncada S. Characterization of the L-arginine:nitric oxide pathway in human platelets. Br J Pharmacol. 1990;101:325-328. https://doi.org/10.1111/j.1476-5381.1990.tb12709.x
  7. Cho EJ, Park MS, Kim SS, Kang G, Choi S, Lee YR, Chang SJ, Lee KH, Lee SD, Park JB, Jeon BH. Vasorelaxing activity of ulmus davidiana ethanol extracts in rats: activation of endothelial nitric oxide synthase. Korean J Physiol Pharmacol. 2011;15:339-344. https://doi.org/10.4196/kjpp.2011.15.6.339
  8. Bernatova I, Pechanova O, Kristek F. Mechanism of structural remodelling of the rat aorta during long-term NG-nitro-L-arginine methyl ester treatment. Jpn J Pharmacol. 1999;81: 99-106. https://doi.org/10.1254/jjp.81.99
  9. Fink J, Fan NY, Rosenfeld L, Stier CT Jr. Contribution of endothelin to the acute pressor response of L-NAME in stroke-prone spontaneously hypertensive rats. J Cardiovasc Pharmacol. 1998;31:618-622. https://doi.org/10.1097/00005344-199804000-00022
  10. Nakanishi K, Mattson DL, Cowley AW Jr. Role of renal medullary blood flow in the development of L-NAME hypertension in rats. Am J Physiol. 1995;268:R317-R323.
  11. Ribeiro MO, Antunes E, de Nucci G, Lovisolo SM, Zatz R. Chronic inhibition of nitric oxide synthesis. a new model of arterial hypertension. Hypertension. 1992;20:298-303. https://doi.org/10.1161/01.HYP.20.3.298
  12. Rees DD, Palmer RM, Moncada S. Role of endothelium-derived nitric oxide in the regulation of blood pressure. Proc Natl Acad Sci U S A. 1989;86:3375-3378. https://doi.org/10.1073/pnas.86.9.3375
  13. Sommerburg O, Keunen JE, Bird AC, van Kuijk FJ. Fruits and vegetables that are sources for lutein and zeaxanthin: the macular pigment in human eyes. Br J Ophthalmol. 1998;82: 907-910. https://doi.org/10.1136/bjo.82.8.907
  14. Curran-Celentano J, Hammond BR Jr, Ciulla TA, Cooper DA, Pratt LM, Danis RB. Relation between dietary intake, serum concentrations, and retinal concentrations of lutein and zeaxanthin in adults in a Midwest population. Am J Clin Nutr. 2001; 74:796-802.
  15. Dwyer JH, Navab M, Dwyer KM, Hassan K, Sun P, Shircore A, Hama-Levy S, Hough G, Wang X, Drake T, Merz CN, Fogelman AM. Oxygenated carotenoid lutein and progression of early atherosclerosis: the Los Angeles atherosclerosis study. Circulation. 2001;103:2922-2927. https://doi.org/10.1161/01.CIR.103.24.2922
  16. Parker RS. Absorption, metabolism, and transport of carotenoids. FASEB J. 1996;10:542-551.
  17. Bhattacharyya S, Datta S, Mallick B, Dhar P, Ghosh S. Lutein content and in vitro antioxidant activity of different cultivars of Indian marigold flower (Tagetes patula L.) extracts. J Agric Food Chem. 2010;58:8259-8264. https://doi.org/10.1021/jf101262e
  18. Kim JH, Na HJ, Kim CK, Kim JY, Ha KS, Lee H, Chung HT, Kwon HJ, Kwon YG, Kim YM. The non-provitamin A carotenoid, lutein, inhibits NF-kappaB-dependent gene expression through redox-based regulation of the phosphatidylinositol 3-kinase/PTEN/Akt and NF-kappaB-inducing kinase pathways: role of H(2)O(2) in NF-kappaB activation. Free Radic Biol Med. 2008;45:885-896. https://doi.org/10.1016/j.freeradbiomed.2008.06.019
  19. Kritchevsky SB, Bush AJ, Pahor M, Gross MD. Serum carotenoids and markers of inflammation in nonsmokers. Am J Epidemiol. 2000;152:1065-1071. https://doi.org/10.1093/aje/152.11.1065
  20. Rowley K, Walker KZ, Cohen J, Jenkins AJ, O'Neal D, Su Q, Best JD, O'Dea K. Inflammation and vascular endothelial activation in an Aboriginal population: relationships to coronary disease risk factors and nutritional markers. Med J Aust. 2003;178:495-500.
  21. Rao AV. Lycopene, tomatoes, and the prevention of coronary heart disease. Exp Biol Med (Maywood). 2002;227:908-913.
  22. Weisburger JH. Lycopene and tomato products in health promotion. Exp Biol Med (Maywood). 2002;227:924-927.
  23. Lee DH, Park JE, Kang YJ, Lee KY, Choi HC. Nitric oxide donor, NOR-3, increased expression of Cyclooxygenase-2, but not of Cyclooxygenase-1 in cultured VSMC. Korean J Physiol Pharmacol. 2006;10:161-165.
  24. Gan XL, Hei ZQ, Huang HQ, Chen LX, Li SR, Cai J. Effect of Astragalus membranaceus injection on the activity of the intestinal mucosal mast cells after hemorrhagic shockreperfusion in rats. Chin Med J (Engl). 2006;119:1892-1898.
  25. Min YS, Lee SE, Hong ST, Kim HS, Choi BC, Sim SS, Whang WK, Sohn UD. The inhibitory effect of quercetin-3-O-beta-Dglucuronopyranoside on gastritis and reflux esophagitis in rats. Korean J Physiol Pharmacol. 2009;13:295-300. https://doi.org/10.4196/kjpp.2009.13.4.295
  26. Hozawa A, Jacobs DR Jr, Steffes MW, Gross MD, Steffen LM, Lee DH. Circulating carotenoid concentrations and incident hypertension: the Coronary Artery Risk Development in Young Adults (CARDIA) study. J Hypertens. 2009;27:237-242. https://doi.org/10.1097/HJH.0b013e32832258c9
  27. Bachmann S, Mundel P. Nitric oxide in the kidney: synthesis, localization, and function. Am J Kidney Dis. 1994;24:112-129.
  28. Zalba G, San Jose G, Moreno MU, Fortuno MA, Fortuno A, Beaumont FJ, Diez J. Oxidative stress in arterial hypertension: role of NAD(P)H oxidase. Hypertension. 2001;38:1395-1399. https://doi.org/10.1161/hy1201.099611
  29. Simko F, Pechanova O, Pelouch V, Krajcirovicova K, Celec P, Palffy R, Bednarova K, Vrankova S, Adamcova M, Paulis L. Continuous light and L-NAME-induced left ventricular remodelling: different protection with melatonin and captopril. J Hypertens. 2010;28 Suppl 1:S13-S18. https://doi.org/10.1097/01.hjh.0000388489.28213.08
  30. De Gennaro Colonna V, Rossoni G, Rigamonti A, Bonomo S, Manfredi B, Berti F, Muller E. Enalapril and quinapril improve endothelial vasodilator function and aortic eNOS gene expression in L-NAME-treated rats. Eur J Pharmacol. 2002;450: 61-66. https://doi.org/10.1016/S0014-2999(02)02046-0
  31. Shesely EG, Maeda N, Kim HS, Desai KM, Krege JH, Laubach VE, Sherman PA, Sessa WC, Smithies O. Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 1996;93:13176-13181. https://doi.org/10.1073/pnas.93.23.13176
  32. John SW, Krege JH, Oliver PM, Hagaman JR, Hodgin JB, Pang SC, Flynn TG, Smithies O. Genetic decreases in atrial natriuretic peptide and salt-sensitive hypertension. Science. 1995; 267:679-681. https://doi.org/10.1126/science.7839143
  33. Iannone A, Rota C, Bergamini S, Tomasi A, Canfield LM. Antioxidant activity of carotenoids: an electron-spin resonance study on beta-carotene and lutein interaction with free radicals generated in a chemical system. J Biochem Mol Toxicol. 1998;12:299-304. https://doi.org/10.1002/(SICI)1099-0461(1998)12:5<299::AID-JBT6>3.0.CO;2-G

Cited by

  1. Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47 phox Expression in Rats vol.7, pp.7, 2015, https://doi.org/10.3390/nu7075222
  2. Effects of Gmelina arborea, Roxb (Verbenaceae) aqueous extract on arterial pressure of Wistar rats vol.7, pp.1, 2013, https://doi.org/10.5897/jpap2015.0099
  3. Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats vol.2017, pp.None, 2013, https://doi.org/10.1155/2017/9460653
  4. Nobiletin alleviates vascular alterations through modulation of Nrf-2/HO-1 and MMP pathways in l-NAME induced hypertensive rats vol.10, pp.4, 2013, https://doi.org/10.1039/c8fo02408a
  5. Anti-atherosclerotic effect of herbal extracts in N(G)-nitro-L-arginine methyl ester-treated rats vol.62, pp.3, 2013, https://doi.org/10.3839/jabc.2019.036
  6. Effect ofHibiscus sabdariffaandZingiber officinaleon the antihypertensive activity and pharmacokinetic of losartan in hypertensive rats vol.50, pp.7, 2013, https://doi.org/10.1080/00498254.2020.1729446
  7. Antihypertensive effect of Phragmanthera incana (Schum) Balle on NG-nitro-L-Arginine methyl ester (L-NAME) induced hypertensive rats vol.257, pp.None, 2020, https://doi.org/10.1016/j.jep.2020.112888
  8. Effects of garden cress, fenugreek and black seed on the pharmacodynamics of metoprolol: an herb-drug interaction study in rats with hypertension vol.59, pp.1, 2013, https://doi.org/10.1080/13880209.2021.1961817
  9. Centella asiatica (L.) Urb. Prevents Hypertension and Protects the Heart in Chronic Nitric Oxide Deficiency Rat Model vol.12, pp.None, 2013, https://doi.org/10.3389/fphar.2021.742562
  10. The effect of swimming training on adrenomedullin levels, oxidative stress variables, and gastrocnemius muscle contractile properties in hypertensive rats vol.43, pp.2, 2021, https://doi.org/10.1080/10641963.2020.1825726
  11. Lutein as a Modulator of Oxidative Stress-Mediated Inflammatory Diseases vol.10, pp.9, 2013, https://doi.org/10.3390/antiox10091448