The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Mechanism of Relaxation Via TASK-2 Channels in Uterine Circular Muscle of Mouse

  • Hong, Seung Hwa (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Sung, Rohyun (Department of Pathology, Chungbuk National University College of Medicine) ;
  • Kim, Young Chul (Department of Physiology, Chungbuk National University College of Medicine) ;
  • Suzuki, Hikaru (Department of Physiology, Nagoya City University Medical School) ;
  • Choi, Woong (Department of Pharmacology, Chungbuk National University College of Medicine) ;
  • Park, Yeon Jin (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Ji, Ill Woon (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Kim, Chan Hyung (Department of Pharmacology, Chungbuk National University College of Medicine) ;
  • Myung, Sun Chul (Department of Urology, College of Medicine, Chung-Ang University) ;
  • Lee, Moo Yeol (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Kang, Tong Mook (Department of Physiology, Sungkyunkwan University School of Medicine) ;
  • You, Ra Young (Department of Physiology, Chungbuk National University College of Medicine) ;
  • Lee, Kwang Ju (Department of Pathology, Chungbuk National University College of Medicine) ;
  • Lim, Seung Woon (Department of Anesthesiology and Pain Medicine, Chungbuk National University College of Medicine) ;
  • Yun, Hyo-Yung (Department of Surgery, Chungbuk National University College of Medicine) ;
  • Song, Young-Jin (Department of Surgery, Chungbuk National University College of Medicine) ;
  • Xu, Wen-Xie (Department of Physiology, Shanghai Jiaotong University School of Medicine) ;
  • Kim, Hak Soon (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Lee, Sang Jin (Department of Physiology, Chungbuk National University College of Medicine)
  • Received : 2013.05.20
  • Accepted : 2013.06.27
  • Published : 2013.08.30
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Abstract

Plasma pH can be altered during pregnancy and at labor. Membrane excitability of smooth muscle including uterine muscle is suppressed by the activation of $K^+$ channels. Because contractility of uterine muscle is regulated by extracellular pH and humoral factors, $K^+$ conductance could be connected to factors regulating uterine contractility during pregnancy. Here, we showed that TASK-2 inhibitors such as quinidine, lidocaine, and extracellular acidosis produced contraction in uterine circular muscle of mouse. Furthermore, contractility was significantly increased in pregnant uterine circular muscle than that of non-pregnant muscle. These patterns were not changed even in the presence of tetraetylammonium (TEA) and 4-aminopyridine (4-AP). Finally, TASK-2 inhibitors induced strong myometrial contraction even in the presence of L-methionine, a known inhibitor of stretch-activated channels in myometrium. When compared to non-pregnant myometrium, pregnant myometrium showed increased immunohistochemical expression of TASK-2. Therefore, TASK-2, seems to play a key role during regulation of myometrial contractility in the pregnancy and provides new insight into preventing preterm delivery.

Keywords

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