The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Effect of Glutamate on the Vestibulo-Solitary Projection after Sodium Nitroprusside-Induced Hypotension in Conscious Rats

  • Li, Li-Wei (Department of Physiology and Pathophysiology, Yanbian University College of Medicine) ;
  • Ji, Guang-Shi (Department of Cerebral Surgery, Yanbian University College of Clinical Medicine) ;
  • Yang, Yan-Zhao (Department of Physiology and Pathophysiology, Yanbian University College of Medicine) ;
  • Ameer, Abdul Nasir (Department of Physiology, Wonkwang University School of Medicine and Brain Science Institute at Wonkwang University) ;
  • Kim, Min Sun (Department of Physiology, Wonkwang University School of Medicine and Brain Science Institute at Wonkwang University) ;
  • Park, Byung Rim (Department of Physiology, Wonkwang University School of Medicine and Brain Science Institute at Wonkwang University) ;
  • Jin, Yuan-Zhe (Department of Physiology and Pathophysiology, Yanbian University College of Medicine)
  • Received : 2015.01.12
  • Accepted : 2015.03.01
  • Published : 2015.05.01
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Abstract

Orthostatic hypotension is most common in elderly people, and its prevalence increases with age. Attenuation of the vestibulo-sympathetic reflex (VSR) is commonly associated with orthostatic hypotension. In this study, we investigated the role of glutamate on the vestibulo-solitary projection of the VSR pathway to clarify the pathophysiology of orthostatic hypotension. Blood pressure and expression of both pERK and c-Fos protein were evaluated in the nucleus tractus solitarius (NTS) after microinjection of glutamate into the medial vestibular nucleus (MVN) in conscious rats with sodium nitroprusside (SNP)-induced hypotension that received baroreceptor unloading via sinoaortic denervation (SAD). SNP-induced hypotension increased the expression of both pERK and c-Fos protein in the NTS, which was abolished by pretreatment with glutamate receptor antagonists (MK801 or CNQX) in the MVN. Microinjection of glutamate receptor agonists (NMDA or AMPA) into the MVN increased the expression of both pERK and c-Fos protein in the NTS without causing changes in blood pressure. These results indicate that both NMDA and AMPA receptors play a significant role in the vestibulo-solitary projection of the VSR pathway for maintaining blood pressure, and that glutamatergic transmission in this projection might play a key role in the pathophysiology of orthostatic hypotension.

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References

  1. Kaufmann H. Consensus statement on the definition of orthostatic hypotension, pure autonomic failure and multiple system atrophy. Clin Auton Res. 1996;6:125-126. https://doi.org/10.1007/BF02291236
  2. Freeman R. Clinical practice. Neurogenic orthostatic hypotension. N Engl J Med. 2008;358:615-624. https://doi.org/10.1056/NEJMcp074189
  3. Yates BJ. Vestibular influences on the sympathetic nervous system. Brain Res Brain Res Rev. 1992;17:51-59. https://doi.org/10.1016/0165-0173(92)90006-8
  4. Doba N, Reis DJ. Role of the cerebellum and the vestibular apparatus in regulation of orthostatic reflexes in the cat. Circ Res. 1974;40:9-18.
  5. Dampney RA, Polson JW, Potts PD, Hirooka Y, Horiuchi J. Functional organization of brain pathways subserving the baroreceptor reflex: studies in conscious animals using immediate early gene expression. Cell Mol Neurobiol. 2003;23:597-616. https://doi.org/10.1023/A:1025080314925
  6. Balaban CD, Beryozkin G. Vestibular nucleus projections to nucleus tractus solitarius and the dorsal motor nucleus of the vagus nerve: potential substrates for vestibulo-autonomic interactions. Exp Brain Res. 1994;98:200-212.
  7. Yates BJ, Grelot L, Kerman IA, Balaban CD, Jakus J, Miller AD. Organization of vestibular inputs to nucleus tractus solitarius and adjacent structures in cat brain stem. Am J Physiol. 1994;267:R974-983.
  8. Lan Y, Yang YZ, Jiang X, Li LW, Jin GS, Kim MS, Park BR, Jin YZ. Additive role of the vestibular end organ and baroreceptors on the regulation of blood pressure in rats. Korean J Physiol Pharmacol. 2013;17:367-373. https://doi.org/10.4196/kjpp.2013.17.4.367
  9. Jiang X, Li LW, Lan Y, Yang YZ, Jin GS, Kim MS, Park BR, Jin YZ. Comparative analysis of vestibular receptor and baroreceptor inputs to the nucleus tractus solitarius following acute hypotension in conscious rats. Neurosci Lett. 2014;563:70-74. https://doi.org/10.1016/j.neulet.2014.01.040
  10. Weston M, Wang H, Stornetta RL, Sevigny CP, Guyenet PG. Fos expression by glutamatergic neurons of the solitary tract nucleus after phenylephrine-induced hypertension in rats. J Comp Neurol. 2003;460:525-541. https://doi.org/10.1002/cne.10663
  11. Park BR, Kim MS, Kim JH, Jin YZ. Effects of acute hypotension on neuronal activity in the medial vestibular nuclei of rats. Neuroreport. 2001;12:3821-3824. https://doi.org/10.1097/00001756-200112040-00044
  12. Kim MS, Hyo Kim J, Kry D, Ae Choi M, Ok Choi D, Gon Cho B, Jin YZ, Ho Lee S, Park BR. Effects of acute hypotension on expression of cFos-like protein in the vestibular nuclei of rats. Brain Res. 2003;962:111-121. https://doi.org/10.1016/S0006-8993(02)03977-X
  13. Choi MA, Lee JH, Hwang JH, Choi SJ, Kim MS, Park BR. Signaling pathway of glutamate in the vestibular nuclei following acute hypotension in rats. Brain Res. 2008;1229:111-117. https://doi.org/10.1016/j.brainres.2008.06.088
  14. Li XL, Nian B, Jin Y, Li LW, Jin GS, Kim MS, Park BR, Jin YZ. Mechanism of glutamate receptor for excitation of medial vestibular nucleus induced by acute hypotension. Brain Res. 2012;1443:27-33. https://doi.org/10.1016/j.brainres.2012.01.020
  15. Li XL, An Y, Jin QH, Kim MS, Park BR, Jin YZ. Changes of some amino acid concentrations in the medial vestibular nucleus of conscious rats following acute hypotension. Neurosci Lett. 2010;477:11-14. https://doi.org/10.1016/j.neulet.2010.04.023
  16. Morgan JI, Curran T. Stimulus-transcription coupling in the nervous system: involvement of the inducible proto-oncogenes fos and jun. Annu Rev Neurosci. 1991;14:421-451. https://doi.org/10.1146/annurev.ne.14.030191.002225
  17. Sgambato V, Pages C, Rogard M, Besson MJ, Caboche J. Extracellular signal-regulated kinase (ERK) controls immediate early gene induction on corticostriatal stimulation. J Neurosci. 1998;18:8814-8825. https://doi.org/10.1523/JNEUROSCI.18-21-08814.1998
  18. Chan RK, Peto CA, Sawchenko PE. Fine structure and plasticity of barosensitive neurons in the nucleus of solitary tract. J Comp Neurol. 2000;422:338-351. https://doi.org/10.1002/1096-9861(20000703)422:3<338::AID-CNE2>3.0.CO;2-2
  19. Wei S, Lei M, Tong M, Ding J, Han Q, Xiao M. Acute baroreceptor unloading evokes Fos expression in anesthetized rat brain. Brain Res Bull. 2008;76:63-69. https://doi.org/10.1016/j.brainresbull.2007.12.003
  20. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. Orlando, FL, USA: Academic Press; 2007.
  21. Kim MS, Choi DO, Choi MA, Kim JH, Kim KY, Lee MY, Rhee JK, Chun SW, Park BR. Immunohistochemical detection of phosphorylated form of extracellular signal-regulated kinase 1/2 in rat vestibular nuclei following hemorrhagic hypotension. Neurosci Lett. 2004;360:49-52. https://doi.org/10.1016/j.neulet.2004.02.023
  22. Jiang X, Lan Y, Jin YZ, Park JY, Park BG, Ameer AN, Park BR. Effect of vestibulosympathetic reflex and baroreflex on expression of pERK in the nucleus tractus solitarius following acute hypotension in conscious rats. Korean J Physiol Pharmacol. 2014;18:353-358. https://doi.org/10.4196/kjpp.2014.18.4.353
  23. Kalia M, Mesulam MM. Brain stem projections of sensory and motor components of the vagus complex in the cat: II. Laryngeal, tracheobronchial, pulmonary, cardiac, and gastrointestinal branches. J Comp Neurol. 1980;193:467-508. https://doi.org/10.1002/cne.901930211
  24. Holstein GR, Friedrich VL Jr, Martinelli GP. Projection neurons of the vestibulo-sympathetic reflex pathway. J Comp Neurol. 2014;522:2053-2074. https://doi.org/10.1002/cne.23517
  25. Murad F. Cyclic guanosine monophosphate as a mediator of vasodilation. J Clin Invest. 1986;78:1-5. https://doi.org/10.1172/JCI112536
  26. Kerman IA, Yates BJ. Regional and functional differences in the distribution of vestibulosympathetic reflexes. Am J Physiol. 1998;275:R824-835.
  27. Takayama K, Suzuki T, Miura M. The comparison of effects of various anesthetics on expression of Fos protein in the rat brain. Neurosci Lett. 1994;176:59-62. https://doi.org/10.1016/0304-3940(94)90871-0

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