Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지
DOI QR코드

DOI QR Code

Sleep-promoting and Anti-anxiety Effects of Shihogayonggolmoryo-tang in Mice

동물모델에서 시호가용골모려탕(柴胡加龍骨牡蠣湯)의 수면유도 및 항불안 효과

  • Lim, Junsik (College of Korean Medicine, Semyung University) ;
  • Leem, Kanghyun (College of Korean Medicine, Semyung University) ;
  • Kim, Taeyeon (College of Korean Medicine, Semyung University)
  • Received : 2020.11.11
  • Accepted : 2021.01.22
  • Published : 2021.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Shihogayonggolmoryo-tang (ST) is a Korean medical herb cocktail which has been used to treat anxiety induced insomnia. In this study, we will examine sleep-promoting and anti-anxiety effects of ST, and investigate its mechanism. ICR mice were divided into three groups for the first examination : control group (n=11), ST50 group (50 mg/kg, po, n=11), ST200 group (200 mg/kg, po, n=11). Sleep-promoting effect was confirmed by measuring the sleeping duration time and sleeping onset time after thiopental sodium treatment (50 mg/kg, ip). ICR mice were divided into five groups for the second examination : control group (n=11), ST200 group (200 mg/kg, po, n=11), ST200+Flumazenil group (ST 200 mg/kg, po, flumazenil 0.3 mg/kg, ip, n=11), diazepam group (1 mg/kg, ip, n=11), diazepam+flumazenil group (diazepam 1 mg/kg, ip, Flumazenil 0.3 mg/kg, ip, n=11). Anxiety behavior and sleep-promoting effect was confirmed by open field test and measuring the sleeping duration time and sleeping onset time. Expression levels of c-fos in tuberomammillary nucleus (TMN) and ventrolateral preoptic nucleus (VLPO) were analyzed by immunohistochemistry. ST treated group showed significantly decreased anxiety behavior and enhanced sleeping duration time and sleeping onset time concentration dependently. The expression of c-fos was significantly upregulated in VLPO as sleep-inducing center and TMN as downregulated in arousal center by ST treatment. In addition, all effects of ST were reversed by flumazenil. Our results suggest that ST has sleep-promoting and anti-anxiety effects through regulating arousal center (TMN) and sleep-inducing center (VLPO).

Keywords

References

  1. Zisapel N. Drugs for insomnia. Expert Opin Emerg Drugs. 2012;17:299-317. https://doi.org/10.1517/14728214.2012.690735
  2. Kim L, Choi YK, Suh KY. The Knowledge and Practice of Sleep Hygiene in Residents of Seoul. J Korean Neuropsychiatr Assoc. 1997;36:712.
  3. Zeitzer JM, Dijk DJ, Kronauer R, Brown E, Czeisler C. Sensitivity of the human circadian pacemaker to nocturnal light: melatonin phase resetting and suppression. J Physiol. 2000;526:695-702. https://doi.org/10.1111/j.1469-7793.2000.00695.x
  4. Yang CK. Pharmacological Treatment of Insomnia. J Korean Society of Biological therapes in Psychiatry. 4, 1998;p. 106.
  5. Greenblatt DJ and Roth T. Zolpidem for insomnia. Expert Opin Pharmacother. 2012;13:879-93. https://doi.org/10.1517/14656566.2012.667074
  6. Besnard S, Masse F, Verdaguer M, Cappelin B, Meurice JC, and Gestreau C. Time- and dose-related effects of three 5-HT receptor ligands on the genioglossus activity in anesthetized and conscious rats. Sleep Breath. 2007;11:275-84. https://doi.org/10.1007/s11325-007-0107-0
  7. Haefely W. Benzodiazepine interactions with GABA receptors. Neuroscience Letters. 1984;47;201-6. https://doi.org/10.1016/0304-3940(84)90514-7
  8. Morin AK. Strategies for treating chronic insomnia. Am J Manag Care. 2006;12:S230-45.
  9. Zammit G. Comparative tolerability of newer agents for insomnia. Drug Saf 2009;32:735-48. https://doi.org/10.2165/11312920-000000000-00000
  10. Moon JJ, An GS, Kim JB, Park WS, Park JH, Kim DH et al. Precise Interpretation of 'Treatise on Cold Damage Diseases'. Yongin:Hanuimunhwasa. 2017:p. 239-40.
  11. Kim SO. The Effect of Shiho Ka Yongol MoryoTang on the Contents of Total Cholesterol, Triglyceride and Phospholipid in the Serum of Rabbit. Kyunghee College of Pharmacy collection of dissertations. 1978;6(1):17-22.
  12. Kim HY, Lee TH. Effect of Sihogayonggolmoryeotang on SPS-induced PTSD in Rats. Herbal formula science. 2019;27(2):121-36. https://doi.org/10.14374/HFS.2019.27.2.121
  13. Song U, Kim SH. The Effects of Kakamsihokayongkolmoryeotang on the Hyperlipidemia. The Journals of the Korean Oriental Medical Society. 1993;26:332-47.
  14. Lee SJ. The operation of Shihogayonggolmoryo-tang on the nervous disease. The Journals of the Korean Oriental Medical Society. 1976;13(1):86-8.
  15. Seo SH, Yim HJ, Jung IC, Lee SR. The Clinical study on 1 case of Patient with Tremor by Bok-Jin. Institute of Oriental Medinine Daejeon University collection of dissertations. 2007;16(1):69-79.
  16. Lee JH, Lee JS, Chung SH. A Clinical Report about Meralgia paresthtica Treated with Sihogayonggolmoryo-tang. The Journals of Oriental Rehab Med. 2002;12(3):157-67.
  17. Lee BY, Lee HJ, Kim JW, Choi HY, Kim YJ, Cho SY, Park SU, Ko CN, Park JM. The Efficacy of Shihogayonggolmoryo-tang in Combating Excessive Sweating in Heart Disease Syndrome, Called 心病 in Korean Medicine. J. Int. Korean Med. 2016;37(4):601-8. https://doi.org/10.22246/jikm.2016.37.4.601
  18. Choi WC, Park DM, Kang WC, Lee SR, Jung IC. Interim Report about The Effect of Sihogayonggolmoryeo-tang on the Anxiety of Hwa-byung. J of Oriental Neuropsychiatry 2012:23(4):133-52. https://doi.org/10.7231/jon.2012.23.4.133
  19. Lee YH. 5 Cases Report of Climacteric Symptoms with Shihogayonggolmoryo-tang. The journal of oriental obstetrics & gynecology. 2013;26(1):121-34. https://doi.org/10.15204/jkobgy.2013.26.1.121
  20. Kim KS, Kim WC, Kim KO. A Study about trends of using Shihogayong-golm-oryo-tang granule Shihogayonggolmoryo-tang, Herbal extracts, non-prescription medicine, prescription medicine. The Korean journal of oriental medical prescription. 2013;20(1):159-66.
  21. Oh DR, Kim Y, Jo A, Im S, Kim CE, Jung MA, Shin J, Kang H, Choi EJ, Kim J, Choi C. Antidepressant-like and Hypnotic Effects of the Herbal Extract Combination of Stauntonia hexaphylla and Vaccinium bracteatum Fruit in Mice. J Physiol & Pathol Korean Med. 2020;34(2):88-96. https://doi.org/10.15188/kjopp.2020.04.34.2.88
  22. Kim HR, Kwon YK, Choi BK, Jung HJ, Baek DG. Effect of Mixture of Atractylodes macrocephala and Amomum villosum Extracts on Body Weight and Lipid Metabolism in High Fat Diet-Induced Obesity Model. J Physiol & Pathol Korean Med. 2020;34(2):75-80. https://doi.org/10.15188/kjopp.2020.04.34.2.75
  23. Jeong HY, Kwon YJ, Park IJ, Kwon JT, Han BH, Lee SP. Effects of the Sanjoin on the Rat Brain. Korean Journal of Biological Psychiatry. 1997;4(1):54-9.
  24. Hua R, Yu S, Liu M and Li H. A PCR-Based Method for RNA Probes and Applications in Neuroscience. Front. Neurosci. 2018;12:266. https://doi.org/10.3389/fnins.2018.00266
  25. Koprdova R, Osacka J, Mach M and Kiss A. Acute Impact of Selected Pyridoindole Derivatives on Fos Expression in Different Structures of the Rat Brain. Cellular and Molecular Neurobiology. 2018;38:171-80. https://doi.org/10.1007/s10571-017-0520-2
  26. Shin YI, Kim SH. GuksiShanghanlun. Seoul:Jumin Publisher. 2006:133-6.
  27. Lobo IA, Harris RA. GABAA receptors and alcohol. Pharmacol Biochem Behav. 2008;90(1):90-4. https://doi.org/10.1016/j.pbb.2008.03.006
  28. Malenka RC, Nestler EJ, Hyman SE. "Chapter 6: Widely Projecting Systems: Monoamines, Acetylcholine, and Orexin". In Sydor A, Brown RY (eds.). Molecular Neuropharmacology: A Foundation for Clinical Neuroscience. 2nd ed. New York:McGraw-Hill Medical. 2009: 175-6.

Cited by

  1. 루이소체 치매로 추정되는 이차성 파킨슨증 환자의 Non-Motor Symptom Scale(NMSS)로 평가한 비운동성 증상을 한약과 침의 복합치료로 호전시킨 증례보고 1례 vol.42, pp.5, 2021, https://doi.org/10.22246/jikm.2021.42.5.833