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Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines

  • Vu, Thi Hao (Department of Animal Science and Technology, Chung-Ang University) ;
  • Hong, Yeojin (Department of Animal Science and Technology, Chung-Ang University) ;
  • Truong, Anh Duc (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Lee, Jiae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Lee, Sooyeon (Department of Animal Science and Technology, Chung-Ang University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University) ;
  • Cha, Jihye (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Dang, Hoang Vu (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Tran, Ha Thi Thanh (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Lillehoj, Hyun S. (Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture) ;
  • Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University)
  • 투고 : 2021.04.08
  • 심사 : 2021.06.07
  • 발행 : 2022.03.01

초록

Objective: The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection. Methods: Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing. Results: In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthase-like, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens. Conclusion: Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.

키워드

과제정보

We thank Department of Biochemistry and Immunology in the National Institute of Veterinary Research, Vietnam for performing animal experiments and collecting samples.

참고문헌

  1. Alexander DJ. An overview of the epidemiology of avian influenza. Vaccine 2007;25:5637-44. https://doi.org/10.1016/j.vaccine.2006.10.051
  2. Chapter O. 10.4. Infection with avian influenza viruses [Internet]. Paris, France: World Organisation for Animal Health; c2001 [2021 July 19]. In: Terrestrial animal health code. https://www.oie.int/fileadmin/Home/eng/Health_standards/tahc/current/chapitre_avian_influenza_viruses.pdf
  3. de Jong JC, Claas ECJ, Osterhaus ADME, Webster RG, Lim WL. A pandemic warning? Nature 1997;389:554. https://doi.org/10.1038/39218
  4. Peiris JM, De Jong MD, Guan Y. Avian influenza virus (H5N1): a threat to human health. Clin Microbiol Rev 2007;20:243-67. https://doi.org/10.1128/CMR.00037-06
  5. Staeheli P, Pitossi F, Pavlovic J. Mx proteins: GTPases with antiviral activity. Trends Cell Biol 1993;3:268-72. https://doi.org/10.1016/0962-8924(93)90055-6
  6. Seyama T, Ko J, Ohe M, et al. Population research of genetic polymorphism at amino acid position 631 in chicken Mx protein with differential antiviral activity. Biochem Genet 2006;44:432-43. https://doi.org/10.1007/s10528-006-9040-3
  7. Jin Y-C, Wei P, Wei X-X, Zhao Z-Y, Li Y. Marek's disease resistant/susceptible MHC haplotypes in Xiayan chickens identified on the basis of BLB2 PCR-RFLP and BLB2/BF2 sequence analyses. Br Poult Sci 2010;51:530-9. https://doi.org/10.1080/00071668.2010.508489
  8. Macklin KS, Ewald SJ, Norton RA. Major histocompatibility complex effect on cellulitis among different chicken lines. Avian Pathol 2002;31:371-6. https://doi.org/10.1080/03079450220141642
  9. Boonyanuwat K, Thummabutra S, Sookmanee N, Vatchavalkhu V, Siripholvat V. Influences of major histocompatibility complex class I haplotypes on avian influenza virus disease traits in Thai indigenous chickens. Anim Sci J 2006;77:285-9. https://doi.org/10.1111/j.1740-0929.2006.00350.x
  10. De Jong MD, Simmons CP, Thanh TT, et al. Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia. Nat Med 2006;12:1203-7. https://doi.org/10.1038/nm1477
  11. Saito LB, Diaz-Satizabal L, Evseev D, et al. IFN and cytokine responses in ducks to genetically similar H5N1 influenza A viruses of varying pathogenicity. J Gen Virol 2018;99:464-74. https://doi.org/10.1099/jgv.0.001015
  12. Ranaware PB, Mishra A, Vijayakumar P, et al. Genome wide host gene expression analysis in chicken lungs infected with avian influenza viruses. PLoS One 2016;11:e0153671. https://doi.org/10.1371/journal.pone.0153671
  13. Wang Y, Lupiani B, Reddy SM, Lamont SJ, Zhou H. RNA-seq analysis revealed novel genes and signaling pathway associated with disease resistance to avian influenza virus infection in chickens. Poult Sci 2014;93:485-93. https://doi.org/10.3382/ps.2013-03557
  14. Hong Y, Truong AD, Lee J, et al. Exosomal miRNA profiling from H5N1 avian influenza virus-infected chickens. Vet Res 2021;52:36. https://doi.org/10.1186/s13567-021-00892-3
  15. Huprikar J, Rabinowitz S. A simplified plaque assay for influenza viruses in Madin-Darby kidney (MDCK) cells. J Virol Methods 1980;1:117-20. https://doi.org/10.1016/0166-0934(80)90020-8
  16. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  17. Yong Y-H, Liu S-F, Hua G-H, et al. Goose toll-like receptor 3 (TLR3) mediated IFN-γ and IL-6 in anti-H5N1 avian influenza virus response. Vet Immunol Immunopathol 2018;197:31-8. https://doi.org/10.1016/j.vetimm.2018.01.010
  18. Abdul-Cader MS, Ahmed-Hassan H, Amarasinghe A, et al. Toll-like receptor (TLR) 21 signalling-mediated antiviral response against avian influenza virus infection correlates with macrophage recruitment and nitric oxide production. J Gen Virol 2017;98:1209-23. https://doi.org/10.1099/jgv.0.000787
  19. Wei L, Jiao P, Yuan R, et al. Goose toll-like receptor 7 (TLR7), myeloid differentiation factor 88 (MyD88) and antiviral molecules involved in anti-H5N1 highly pathogenic avian influenza virus response. Vet Immunol Immunopathol 2013;153:99-106. https://doi.org/10.1016/j.vetimm.2013.02.012
  20. Barjesteh N, Abdelaziz KT, Sharif S. The role of IRF7 and NF-κB pathways in the induction of antiviral responses in chicken tracheal epithelial cells following exposure to TLR3 and 4 ligands. J Immunol 2016;196 (Suppl 1):216.8.
  21. Nturibi E, Bhagwat AR, Coburn S, Myerburg MM, Lakdawala SS. Intracellular colocalization of influenza viral RNA and Rab11A is dependent upon microtubule filaments. J Virol 2017;91:e01179-17. https://doi.org/10.1128/JVI.01179-17
  22. Cheung C, Poon L, Lau A, et al. Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? The Lancet 2002;360:1831-7. https://doi.org/10.1016/S0140-6736(02)11772-7
  23. Salomon R, Hoffmann E, Webster RG. Inhibition of the cytokine response does not protect against lethal H5N1 influenza infection. Proc Natl Acad Sci USA 2007;104:12479-81. https://doi.org/10.1073/pnas.0705289104
  24. De Silva Senapathi U, Abdul-Cader MS, Amarasinghe A, et al. The in ovo delivery of CpG oligonucleotides protects against infectious bronchitis with the recruitment of immune cells into the respiratory tract of chickens. Viruses 2018;10:635. https://doi.org/10.3390/v10110635
  25. Rong E, Wang X, Chen H, et al. Molecular mechanisms for the adaptive switching between the OAS/RNase L and OASL/RIG-I pathways in birds and mammals. Front Immunol 2018;9:1398. https://doi.org/10.3389/fimmu.2018.01398
  26. Garcia MA, Gil J, Ventoso I, et al. Impact of protein kinase PKR in cell biology: from antiviral to antiproliferative action. Microbiol Mol Biol Rev 2006;70:1032-60. https://doi.org/10.1128/MMBR.00027-06
  27. Rohaim MA, Santhakumar D, Naggar RFE, et al. Chickens expressing IFIT5 ameliorate clinical outcome and pathology of highly pathogenic avian influenza and velogenic newcastle disease viruses. Front Immunol 2018;9:2025. https://doi.org/10.3389/fimmu.2018.02025
  28. Wang X, Hinson ER, Cresswell P. The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell Host Microbe 2007;2:96-105. https://doi.org/10.1016/j.chom.2007.06.009
  29. Forster R, Davalos-Misslitz AC, Rot A. CCR7 and its ligands: balancing immunity and tolerance. Nat Rev Immunol 2008;8:362-71. https://doi.org/10.1038/nri2297
  30. Tag-EL-Din-Hassan HT, Morimatsu M, Agui T. Functional analysis of duck, goose, and ostrich 2'-5'-oligoadenylate synthetase. Infect Genet Evol 2018;62:220-32. https://doi.org/10.1016/j.meegid.2018.04.036
  31. Pichlmair A, Lassnig C, Eberle C-A, et al. IFIT1 is an antiviral protein that recognizes 5'-triphosphate RNA. Nat Immunol 2011;12:624-30. https://doi.org/10.1038/ni.2048