동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제36권6호
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  • Pages.209-212
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  • 2022
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
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산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과

Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells

  • 권강범 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) ;
  • 김하림 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) ;
  • 김예슬 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) ;
  • 박은희 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) ;
  • 최한별 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오)
  • Kang Beom, Kwon (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.) ;
  • Ha Rim, Kim (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.) ;
  • Ye Seul, Kim (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.) ;
  • Eun Hee, Park (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.) ;
  • Han Byeol, Choi (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.) ;
  • Do Gon, Ryu (Department of Physiology, School of Korean Medicine, Wonkwang University & Ilwonbio Co., Ltd.)
  • 투고 : 2022.11.23
  • 심사 : 2022.12.22
  • 발행 : 2022.12.25
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초록

We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H2O2)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H2O2, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H2O2 groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H2O2 for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H2O2. But GEE suppressed H2O2-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H2O2. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.

키워드

과제정보

본 연구는 2022년도 원광대학교 교비 지원에 의해 수행됨.

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