동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제36권6호
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  • Pages.235-241
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  • 2022
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
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사삼생맥산 추출물이 Balb/c mice의 면역활성에 미치는 효과

Effect of Sasamsaengmaek-san Extract on Immunity Activity in Balb/c Mice

  • 박채현 (대전대학교 한의과대학 침구학교실) ;
  • 강재희 (대전대학교 한의과대학 침구학교실) ;
  • 이현 (대전대학교 한의과대학 침구학교실)
  • Chae Hyun, Park (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Daejeon University) ;
  • Jae Hui, Kang (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Daejeon University) ;
  • Hyun, Lee (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Daejeon University)
  • 투고 : 2022.10.07
  • 심사 : 2022.12.27
  • 발행 : 2022.12.25
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초록

The purpose of this study was to investigate the immune activation effect of Sasamsaengmaek-san (SSSMS) consisted of a mixture of Adenophora triphylla var. japonica, Liriope platyphylla, Panax ginseng C. A. Meyer and Schisandra chinensis. in Balb/c mice. Measuring alanine aminotransferase (ALT) and aspartic acid transaminase (AST) levels in Balb/c mice was performed to analyze the cytotoxicity. Cytokines (IFN-γ, IL-2, IL-12) which regulate the immune activation in Balb/c mice were measured by enzyme-linked immunosorbent assay (ELISA). Activated T lymphocytes in peripheral blood mononuclear cell (PBMC), spleen, lymph nodes were analyzed by flow cytometry using percentages. All tests were compared with red ginseng 100 ㎍/mL (RG 100), which is the most used for immune activity. As a result, cytokine activity was significantly increased at SSSMS 300 group. Activated T lymphocytes in PBMC, spleen, lymph nodes were significantly increased at SSSMS 300 group. These results suggest that there is a possibility of SSSMS activating an immune system by activating the cytokines, and it is confirmed that SSSMS also effective for generation and differentiation of T, B lymphocytes which activate the immune response.

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