Animal Bioscience

  • 제35권10호
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  • Pages.1535-1544
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

  • Zhu, Wen (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Liu, Tianwei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Deng, Jian (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wei, Cong Cong (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhang, Zi Jun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wang, Di Ming (Key Laboratory of Molecular Animal Nutirtion, Ministry of Education, Zhejiang University) ;
  • Chen, Xing Yong (College of Animal Science and Technology, Anhui Agricultural University)
  • 투고 : 2021.09.07
  • 심사 : 2022.02.18
  • 발행 : 2022.10.01
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초록

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

키워드

과제정보

This research was funded by the National Key R&D Program of China (No. 2018YFD0502000), Key Laboratory of Molecular Animal Nutrition of Ministry of Education of China, the Science and Technology Major Project of Anhui Province (No. 201903b06020001), National Innovative Training Program for College Student (No. 201910364046), The National Natural Science Foundation of China (No. 32002199), Anhui province Natural Science Foundation of China (No. 2008085MC86), and National Natural Science Foundation of China (No. 32002199).

참고문헌

  1. Kohn RA, Dinneen MM, Russek-Cohen E. Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs, and rats. J Anim Sci 2005;83:879-89. https://doi.org/10.2527/2005.834879x
  2. Wang Y, Cao P, Wang L, Zhao Z, Chen Y, Yang Y. Bacterial community diversity associated with different levels of dietary nutrition in the rumen of sheep. Appl Microbiol Biotechnol 2017;101:3717-28. https://doi.org/10.1007/s00253-017-8144-5
  3. Muscher AS, Wilkens MR, Mrochen N, Schroder B, Breves G, Huber K. Ex vivo intestinal studies on calcium and phosphate transport in growing goats fed a reduced nitrogen diet. Br J Nutr 2012;108:628-37. https://doi.org/10.1017/S0007114511005976
  4. Zhu W, Xu W, Wei CC, Zhang ZJ, Jiang CC, Chen XY. Effects of decreasing dietary crude protein level on growth performance, nutrient digestion, serum metabolites, and nitrogen utilization in growing goat kids (Capra. hircus). Animals 2020;10:151. https://doi.org/1c0.3390/ani10010151 10010151
  5. Agarwal N, Kamra DN, Chaudhary LC. Rumen microbial ecosystem of domesticated ruminants. In: Puniya A, Singh R, Kamra D, editors. Rumen microbiology: From evolution to revolution, New Delhi, India: Springer Press; 2015. https://doi.org/10.1007/978-81-322-2401-3_2
  6. Zhou M, Peng YJ, Chen YH, et al. Assessment of microbiome changes after rumen transfaunation: implications on improving feed efficiency in beef cattle. Microbiome 2018;6:62. https://doi.org/10.1186/s40168-018-0447-y
  7. Hua CF, Tian J, Tian P, et al. Feeding a high concentration diet induces unhealthy alterations in the composition and metabolism of ruminal microbiota and host response in a goat model. Front Microbiol 2017;8:138. https://doi.org/10.3389/fmicb.2017.00138
  8. Clark JH, Davis CL. Some aspects of feeding high producing dairy cows. J Dairy Sci 1980;63:873-85. https://doi. org/10.3168/jds.s0022-0302(80)83021-9
  9. Gao J, Sun YF, Bao Y, Zhou K, Kong DH, Zhao GY. Effects of different levels of rapeseed cake containing high glucosinolates in steer ration on rumen fermentation, nutrient digestibility and the rumen microbial community. Br J Nutr 2021;125:266-74. https://doi.org/10.1017/S0007114520002767
  10. Nicholson JK, Holmes E, Kinross J, et al. Host-gut microbiota metabolic interactions. Science 2012;336:1262-7. https://doi.org/10.1126/science.1223813
  11. National Research Council. Nutrient requirements of small ruminants, sheep, goats, cervids, and new world camelids. Washington, DC, USA: National Academy Press; 2007.
  12. Hu WL, Liu JX, Ye JA, Wu YM, Guo YQ. Effect of tea saponin on rumen fermentation in vitro. Anim Feed Sci Technol 2005;120:333-9. https://doi.org/10.1016/j.anifeedsci.2005.02.029
  13. Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem 1962;8:130-2. https://doi.org/10.1093/clinchem/8.2.130
  14. Wang B, Ma MP, Diao QY, Tu Y. Saponin-induced shifts in the rumen microbiome and metabolome of young cattle. Front Microbiol 2019;10:356. https://doi.org/10.3389/fmicb.2019.00356
  15. Sun HZ, Wang DM, Wang B, et al. Metabolomics of four biofluids from dairy cows: potential bimarkers for milk production and quality. J Proteome Res 2015;14:1287-98. https://doi.org/10.1021/pr501305g
  16. Xia C, Rahman MAU, Yang H, et al. Effect of increased dietary crude protein levels on production performance, nitrogen utilisation, blood metabolites and ruminal fermentation of Holstein bulls. Asian-Australas J Anim Sci 2018;31: 1643-53. https://doi.org/10.5713/ajas.18.0125
  17. Zhou K, Bao Y, Zhao GY. Effects of dietary crude protein and tannic acid on rumen fermentation, rumen microbiota and nutrient digestion in beef cattle. Arch Anim Nutr 2019;73: 30-43. https://doi.org/10.1080/1745039x.2018.1545502
  18. National Research Council. Nutrient requirements of dairy cattle. 7th rev. ed. National Academy of Sciences: Washington, DC, USA: National Academies Press; 2001.
  19. Calsamiglia S, Ferret A, Reynolds CK, Kristensen NB, van Vuuren AM. Strategies for optimizing nitrogen use by ruminants. Animal 2010;4:1184-96. https://doi.org/10.1017/s1751731110000911
  20. Mann E, Wetzels Wagner SUM, Zebeli Q, Schmitz-Esser S. Metatranscriptome sequencing reveals insights into the gene expression and functional potential of rumen wall bacteria. Front Microbiol 2018;9:43. https://doi.org/10.3389/fmicb.2018.00043
  21. Han X, Yang Y, Yan H, Wang X, Qu L, Chen Y. Rumen bacterial diversity of 80 to 110-day-old goats using 16S rRNA sequencing. PLoS One 2015;10:e0117811. https://doi.org/10.1371/journal.pone.0117811
  22. Zhang XX, Li YX, Tang ZR, et al. Reducing protein content in the diet of growing goats: implications for nitrogen balance, intestinal nutrient digestion and absorption, and rumen microbiota. Animal 2020;14:2063-73. https://doi.org/10.1017/S1751731120000890
  23. Zhu W, Su Z, Xu W, et al. Garlic skin induces shifts in the rumen microbiome and metabolome of fattening lambs. Animal 2021;15:100216. https://doi.org/10.21203/rs.3.rs57466/v1
  24. Chanthakhoun V, Wanapat M, Berg J. Level of crude protein in concentrate supplements influenced rumen characteristics, microbial protein synthesis and digestibility in swamp buffaloes (bubalus bubalis). Livest Sci 2012;144:197-204. https://doi.org/10.1016/j.livsci.2011.11.011
  25. Remond B, Souday E, Ouany JP. In vitro and in vivo fermentation of glycerol by rumen microbes. Anim Feed Sci Technol 1993;41:121-32. https://doi.org/10.1016/0377-8401(93)901 18-4
  26. Garrido-Franco M. Pyridoxine 5'-phosphate synthase: de novo synthesis of vitamin B6 and beyond. Biochim Biophys Acta Proteins Proteom 2003;1647:92-7. https://doi.org/10.1016/s1570-9639(03)00065-7
  27. Gholizadeh M, Fayazi J, Asgari Y, Zali H, Kaderali L. Reconstruction and analysis of cattle metabolic networks in normal and acidosis rumen tissue. Animal 2020;10:469. https://doi.org/10.3390/ani10030469
  28. Kelsay J, Baysal A, Linkswiler H. Effect of vitamin B6 depletion on the pyridoxal, pyridoxamine and pyridoxine content of the blood and urine of men. J Nutr 1968;94:490-4. https://doi.org/10.1093/jn/94.4.490
  29. Meale SJ, Morgavi DP, Cassar-Malek I, et al. Exploration of biological markers of feed efficiency in young bulls. J Agric Food Chem 2017;65:9817-27. https://doi.org/10.1021/acs.jafc.7b03503