The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Antioxidation and anti-inflammatory effects of gamma-irradiated silk sericin and fibroin in H2O2-induced HaCaT Cell

  • Ji-Hye Choi (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University) ;
  • Sangmin Lee (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University) ;
  • Hye-Ju Han (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University) ;
  • Jungkee Kwon (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University)
  • Received : 2022.08.31
  • Accepted : 2022.10.25
  • Published : 2023.01.01
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Abstract

Oxidative stress in skin cells can induce the formation of reactive oxygen species (ROS), which are critical for pathogenic processes such as immunosuppression, inflammation, and skin aging. In this study, we confirmed improvements from gamma-irradiated silk sericin (I-sericin) and gamma-irradiated silk fibroin (I-fibroin) to skin cells damaged by oxidative stress. We found that I-sericin and I-fibroin effectively attenuated oxidative stress-induced ROS generation and decreased oxidative stress-induced inflammatory factors COX-2, iNOS, tumor necrosis factor-α, and interleukin-1β compared to the use of non-irradiated sericin or fibroin. I-sericin and Ifibroin effects were balanced by competition with skin regenerative protein factors reacting to oxidative stress. Taken together, our results indicated that, compared to non-irradiated sericin or fibroin, I-sericin, and I-fibroin had anti-oxidation and antiinflammation activity and protective effects against skin cell damage from oxidative stress. Therefore, gamma-irradiation may be useful in the development of cosmetics to maintain skin health.

Keywords

Acknowledgement

This research was supported by "Research Base Construction Fund Support Program" funded by Jeonbuk National University in 2020.

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