Animal Bioscience

  • 제36권8호
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  • Pages.1199-1208
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  • 2023
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis

  • Hyun Sang Kim (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jun Sik Eom (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Shin Ja Lee (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Youyoung Choi (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Seong Uk Jo (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Sang Suk Lee (Ruminant Nutrition and Anaerobe Laboratory, College of Bio-industry Science, Sunchon National University) ;
  • Eun Tae Kim (National Institute of Animal Science, Rural Development Administration) ;
  • Sung Sill Lee (Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2022.12.27
  • 심사 : 2023.01.31
  • 발행 : 2023.08.01
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초록

Objective: The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods: Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegrass and 20% concentrate of daily intake of dry matter) group and a high concentrate diet (HC; 20% Italian ryegrass and 80% concentrate) group. Blood was collected from the jugular vein, and milk was sampled using a milking machine. Metabolite levels in serum and milk were estimated using proton nuclear magnetic resonance and subjected to qualitative and quantitative analyses performed using Chenomx 8.4. For statistical analysis, Student's t-test and multivariate analysis were performed using Metaboanalyst 4.0. Results: In the principal component analysis, a clear distinction between the two groups regarding milk metabolites while serum metabolites were shown in similar. In serum, 95 metabolites were identified, and 13 metabolites (include leucine, lactulose, glucose, betaine, etc.) showed significant differences between the two groups. In milk, 122 metabolites were identified, and 20 metabolites (include urea, carnitine, acetate, butyrate, arabinitol, etc.) showed significant differences. Conclusion: Our results show that different concentrate to forage feed ratios impact the metabolite levels in the serum and milk of lactating Holstein cows. A higher number of metabolites in milk, including those associated with milk fat synthesis and the presence of Escherichia coli in the rumen, differed between the two groups compared to that in the serum. The results of this study provide a useful insight into the metabolites associated with different concentrate to forge feed ratios in cows and may aid in the search for potential biomarkers for subacute ruminal acidosis.

키워드

과제정보

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) Through Livestock Industrialization Technology Development Program, Funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(321083052HD020).

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