Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

  • Wenzheng Shen (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yu Fu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Haiyu Bai (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhiyu Zhang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhikun Cao (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zibo Liu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Chao Yang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Shixin Sun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Lei Wang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Chunhuan Ren (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yinghui Ling (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zijun Zhang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Hongguo Cao (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2023.08.30
  • Accepted : 2023.11.28
  • Published : 2024.04.01
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Abstract

Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

Keywords

Acknowledgement

This work was supported by National Key Research and Development Program of China (2022YFD1300202), Anhui Key Research and Development Program (2023z04020003; 2023j11020001) National Natural Science Foundation of China (32272875), and Anhui Natural Science Foundation (2208085MC75).

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