Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Baicalein inhibits IL-1β-induced extracellular matrix degradation with decreased MCP-1 expression in primary rat chondrocytes

  • InA Cho (Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University) ;
  • Ki-Ho Chung (Department of Preventive and Public Health Dentistry, School of Dentistry, Chonnam National University) ;
  • Young Kim (Hard-Tissue Biointerface Research Center, School of Dentistry, Chonnam National University) ;
  • Choong-Ho Choi (Department of Preventive and Public Health Dentistry, School of Dentistry, Chonnam National University) ;
  • Jeong-Tae Koh (Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University)
  • Received : 2023.08.28
  • Accepted : 2024.01.05
  • Published : 2024.04.15
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Abstract

Baicalein is a flavonoid extracted from the roots of Scutellaria baicalensis and Scutellaria lateriflora. This compound exerts various biochemical activities, including antioxidant and anti-inflammatory effects. The study aimed to investigate the effect of baicalein on articular cartilage cells and elucidate its underlying mechanism. In primary rat chondrocyte cultures, treatment with baicalein demonstrated a reduction in the loss of proteoglycan and extracellular matrix degradation induced by interleukin (IL)-1β. Baicalein suppressed IL-1β-induced catabolic responses, including the expression and activation of matrix metalloproteinase (MMP)-13, MMP-3, and MMP-1. In addition, baicalein effectively reduced nitric oxide and prostaglandin E2 production, and it downregulated the expression of inducible nitric oxide synthase and cyclooxygenase-2 in primary rat chondrocytes. Furthermore, baicalein downregulated IL-1β-induced inflammatory chemokines and cytokines, such as GM-CSF and MCP-1. These findings suggest that baicalein could potentially mitigate the catabolic responses of IL-1β in chondrocytes, making it a promising candidate for both the prevention and treatment of osteoarthritis.

Keywords

Acknowledgement

The authors would like to extend their sincere thanks to Suk-Gyun Park and Seung Hee Kwon for their technical assistance.

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