Animal Bioscience

  • 제37권5호
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  • Pages.929-943
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  • 2024
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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RNA-Seq explores the functional role of the fibroblast growth factor 10 gene in bovine adipocytes differentiation

  • Nurgulsim Kaster (College of Animal Science and Technology, Northwest A&F University) ;
  • Rajwali Khan (College of Animal Science and Technology, Northwest A&F University) ;
  • Ijaz Ahmad (Department of Livestock Management, Breeding and Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar) ;
  • Kazhgaliyev Nurlybay Zhigerbayevich (Candidate of Sciences in Agriculture, Researcher of Scientific and Production Centre for Animal Husbandry and Veterinary Limited Liability Partnership) ;
  • Imbay Seisembay (Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro Technical University) ;
  • Akhmetbekov Nurbolat (Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro Technical University) ;
  • Shaikenova Kymbat Hamitovna (Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro Technical University) ;
  • Omarova Karlygash Mirambekovna (Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro Technical University) ;
  • Makhanbetova Aizhan Bekbolatovna (Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro Technical University) ;
  • Tlegen Garipovich Amangaliyev (Zhengir Khan West Kazakhstan Agrarian and Technical University) ;
  • Ateikhan Bolatbek (Faculty of Agricultural Sciences, Toraighyrov University) ;
  • Titanov Zhanat Yeginbaevich (Faculty of Agricultural Sciences, Toraighyrov University) ;
  • Shakoor Ahmad (College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar) ;
  • Zan Linsen (College of Animal Science and Technology, Northwest A&F University) ;
  • Begenova Ainagul Baibolsynovna (College of Animal Science and Technology, Northwest A&F University)
  • 투고 : 2023.05.17
  • 심사 : 2023.09.18
  • 발행 : 2024.05.01
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초록

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results: The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p<0.05) reduced the lipid droplets and TG content, and their down-regulation (siFGF10) increased the measurement of lipid droplets and TG in differentiated bovine adipocytes. Furthermore, the overexpression of the FGF10 gene down regulated the mRNA levels of adipogenic marker genes such as CCAAT enhancer binding protein alpha (C/EBPα), fatty acid binding protein (FABP4), peroxisome proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), and Fas cell surface death receptor (FAS), similarly, down-regulation of the FGF10 gene enriched the mRNA levels of C/EBPα, PPARγ, FABP4, and LPL genes (p<0.01). Additionally, the protein levels of PPARγ and FABP4 were reduced (p<0.05) in adipocytes infected with Ad-FGF10 gene and enriched in adipocytes transfected with siFGF10. Moreover, a total of 1,774 differentially expressed genes (DEGs) including 157 up regulated and 1,617 down regulated genes were explored in adipocytes infected with Ad-FGF10 or Ad-NC through deep RNA-sequencing. The top Kyoto encyclopedia of genes and genomes pathways regulated through DEGs were the PPAR signaling pathway, cell cycle, base excision repair, DNA replication, apoptosis, and regulation of lipolysis in adipocytes. Conclusion: Therefore, we can conclude that the FGF10 gene is a negative regulator of bovine adipogenesis and could be used as a candidate gene in marker-assisted selection.

키워드

과제정보

This study was funded by the National Natural Science Foundation of China (31972994), the National Beef and Yak Industrial Technology System (CARS-37), the Key Research and Development Program of Shaanxi Province (2022ZDLNY01-01).

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