Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effect of Resveratrol on Lipopolysaccharide-induced p21 (WAF1/CIP1) and Bax Expression in Astroglioma C6 Cells

C6 신경교세포에서 lipopolysaccharide에 의한 p21 (WAF1/CIP1) 및 Bax의 발현증가에 미치는 resveratrol의 영향

  • Kim, Young-Ae (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University) ;
  • Lim, Sun-Young (Division of Ocean Science, Korea Maritime University) ;
  • Rhee, Sook-Hee (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Biomedical Research Center of Oriental Medicine)
  • 김영애 (일본 부산의과약과대학 화한약연구소) ;
  • 임선영 (한국해양대학교 해양과학기술대학 해양과학부) ;
  • 이숙희 (부산대학교 생활환경대학 식품영양학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 한방바이오연구센터)
  • Published : 2005.02.25
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Abstract

Resveratrol, a phytoalexin found at high levels in grapes and in grape products such as red wine, has been reported to possess a wide range of biological and pharmacological activities including anti-oxident, anti-inflammatory, anti-mutagenic, and anti-carcinogenic effects, but its molecular mechanism is poorly understood. In this study, we examined the effects of resveratrol on lipopolysaccharide (LPS)-induced growth inhibitory activity and cell growth-regulatory gene products in astroglioma C6 cells to elucidate its possible mechanism for anti-cytotoxicity. It is shown that LPS induced time-dependent growth inhibition and morphological changes of C6 cells, which were recovered by pre-treatment with resveratrol. The anti-proliferative effect of LPS was associated with the induction of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21 (WAF1/CIP1) expression assessed by RT-PCR and Western blot analysis in time-dependent manner in C6 cells. In addition, the pro-apoptotic Bax expression was also up-regulated in LPS-treated C6 cells without alteration of anti-apoptotic Bcl-2 and Bcl-XL expression. However, resveratrol significantly inhibited LPS-induced p53, p21 and Bax levels, suggesting that the modulation of p53, p21 and Bax levels could be one of the possible pathways by which resveratrol functions as anti-cytotoxic agent.

Keywords

References

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