The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Protective Effects of the Nuclear Factor Kappa B Inhibitor Pyrrolidine Dithiocarbamate on Experimental Testicular Torsion and Detorsion Injury

  • Kabay, Sahin (Department of Urology, Faculty of Medicine, Dumlupınar University) ;
  • Ozden, Hilmi (Department of Anatomy, Faculty of Medicine, Eskisehir Osmangazi University) ;
  • Guven, Gul (Department of Anatomy, Faculty of Medicine, Eskisehir Osmangazi University) ;
  • Burukoglu, Dilek (Department of Histology and Embryology, Faculty of Medicine, Eskisehir Osmangazi University) ;
  • Ustuner, Mehmet Cengiz (Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University) ;
  • Topal, Fatma (Bilecik Seyh Edebali University, Vocational School Health Services) ;
  • Gunes, Hasan Veysi (Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University) ;
  • Ustuner, Derya (Eskisehir Osmangazi University, Vocational School Health Services) ;
  • Ozbayer, Cansu (Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University)
  • Received : 2014.03.17
  • Accepted : 2014.06.27
  • Published : 2014.08.30
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Abstract

Testicular torsion results with the damage of the testis and it is a surgical emergency. Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight antioxidant and potent inhibitor of nuclear factor kappa B (NF-${\kappa}B$) activation. In this study, we aimed to investigate the effects of PDTC to testicular torsion-detorsion (T/D) injury. Forty adult male Sprague-Dawley rats were separated into four groups. A sham operation was performed in group I. In group II, torsion is performed 2 hours by 720 degree extravaginally testis. In group III, 4 h reperfusion of the testis was performed after 2 h of testicular torsion. In group IV, after performing the same surgical procedures as in group III, PDTC (100 mg/kg, intravenous's) was administered before 30 min of detorsion. The testes tissue malondialdehyde (MDA), superoxide dismutase (SOD) catalase (CAT) level was evaluated. Histological evaluations were performed after hematoxylin and eosin staining. Testicular tissue MDA levels were the highest in the T/D groups compared with treatment group. Administration of PDTC prevented a further increase in MDA levels. Significant decrease occurred in CAT and SOD levels in treatment group compared with the control group. The rats in the treatment group had normal testicular architecture. The results suggest that PDTC can be a potential protective agent for preventing the biochemical and histological changes related to oxidative stress in testicular injury caused by testis torsion.

Keywords

References

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