The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Protective Effect of Phosphatidylcholine on Lipopolysaccharide-Induced Acute Inflammation in Multiple Organ Injury

  • Jung, Yoon Yang (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Nam, Yunsung (Department of Pharmacology, College of Medicine, Chung-Ang University) ;
  • Park, Yong Seol (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Lee, Ho Sung (Department of Pharmacology, College of Medicine, Chung-Ang University) ;
  • Hong, Soon Auck (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Kim, Beom Keun (Department of Pharmacology, College of Medicine, Chung-Ang University) ;
  • Park, Eon Sub (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Chung, Yoon Hee (Department of Anatomy, College of Medicine, Chung-Ang University) ;
  • Jeong, Ji Hoon (Department of Pharmacology, College of Medicine, Chung-Ang University)
  • Received : 2013.04.04
  • Accepted : 2013.04.22
  • Published : 2013.06.30
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Abstract

Soybean polyunsaturated phosphatidylcholine (PC) is thought to exert anti-inflammatory activities and has potent effects in attenuating acute renal failure and liver dysfunction. The aim of this study was to investigate the effects of PC in protecting multiple organ injury (MOI) from lipopolysaccharide (LPS). Six groups of rats (N=8) were used in this study. Three groups acted as controls and received only saline, hydrocortisone (HC, 6 mg/kg, i.v.) or PC (600 mg/kg, i.p.) without LPS (15 mg/kg, i.p.) injections. Other 3 groups, as the test groups, were administered saline, HC or PC in the presence of LPS. Six hours after the LPS injection, blood and organs (lung, liver and kidney) were collected from each group to measure inflammatory cytokines and perform histopathology and myeloperoxidase (MPO) assessment. Serum cytokines (TNF-${\alpha}$, IL-6 and IL-10) and MPO activities were significantly increased, and significant histopathological changes in the organs were observed by LPS challenge. These findings were significantly attenuated by PC or HC. The treatment with PC or HC resulted in a significant attenuation on the increase in serum levels of TNF-${\alpha}$ and IL-6, pro-inflammatory cytokines, while neither PC nor HC significantly attenuated serum levels of IL-10, anti-inflammatory cytokine. In the organs, the enhanced infiltration of neutrophils and expression of ED2 positive macrophage were attenuated by PC or HC. Inductions of MPO activity were also significantly attenuated by PC or HC. From the findings, we suggest that PC may be a functional material for its use as an anti-inflammatory agent.

Keywords

References

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